1. Various strategies for producing transgenic and mutant mice
Various strategies for producing transgenic and mutant mice. DNA can be injected into the male pronucleus of fertilized mouse eggs, followed by implantation in pseudopregnant mothers. Mouse embryos can be infected with retroviruses in vivo or in vitro. Transgenic mice may be mosaic, but the transgene can be recovered in nonmosaic form in the offspring of these mice. These strategies are depicted on the left. On the right, cultured embryonic stem (ES) cells can be modified by homologous or nonhomologous recombination or by retroviral infection. The modified cells can be selected and injected into a mouse blastocyst to produce a chimeric mouse, with subsequent recovery of the mutation in the germ line of the offspring of the chimeric mouse. (From Beaudet AL: Harrison’s Principles of Internal Medicine, 12th ed. New York, McGraw-Hill, 1994, p 349. Used by permission.)
Source: Mouse Models of Human Genetic Disorders, The Online Metabolic and Molecular Bases of Inherited Disease
Citation: Valle D, Beaudet AL, Vogelstein B, Kinzler KW, Antonarakis SE, Ballabio A, Gibson K, Mitchell G. The Online Metabolic and Molecular Bases of Inherited Disease; 2014 Available at: Accessed: November 09, 2017
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