1. Half Adder - Modeling Overview
September 4th, 2006
ETH Zürich | iGEM team 2006

2. System Architecture Half-Adder Light Sensing PoPS Duplexer Light
PoPS to RFP
XOR
PoPS
PoPS to GFP
AND
Chemical Sensing
PoPS Duplexer
PoPS
PoPS
PoPS

3. Insights Overview

4. Light Sensing ho1 PcyA Cph1 Cph1 Cph1 EnvZ EnvZ EnvZ P OmpR P OmpR
Heme
PCB
ho1
PcyA
PCB
PCB
Cph1
Cph1
Cph1
EnvZ
EnvZ
EnvZ P
Auto-Phosphorylation
OmpR P
Part: BBa_I15010
OmpR
Cph8: Fusion Cph1/EnvZ Chimeric Cph1 light receptor/EnvZ protein
Part:BBa_I15008
Heme Oxygenase (ho1)
PoPS
Part:BBa_I15009
Phycocyanobilin (PcyA) ferredoxin oxidoreductase
Part: BBa_R0082

5. Chemical Sensing LacI LacI IPTG IPTG IPTG PoPS Part: BBa_Q01121
BBa_R0011
Strong promoter
(lacI regulated)
BBa_B0012
Terminator
BBa_I14032
Strong constitutive
ON promoter
BBa_B0031
RBS
BBa_C0012
LacI repressor
BBa_B0010
Terminator

6. PoPS Duplexer D1 D2 D3 D4 Light PoPS Duplexer Chemical PoPS Duplexer

7. AND (Version A)
PoPS e1 E1
PoPS E1 E2 E2
PoPS e2

8. AND (Version B)
PoPS a R A
PoPS r1 r2 B
PoPS R b

9. XOR I1
PoPS i1
i2’
I1’
I1 - I1‘
PoPS
I2 - I2‘
PoPS
I2’ i2
i1’ I2

10. PoPS to Reporter
GFP
PoPS
gfp
RFP
PoPS
rfp