1. From: Canonical/β-Catenin Wnt Pathway Activation Improves Retinal Pigmented Epithelium Derivation From Human Embryonic Stem Cells
Invest. Ophthalmol. Vis. Sci ;56(2): doi: /iovs
Figure Legend:
Fluorescent reporters reflect endogenous gene expression during directed differentiation to RPE. (A) Fluorescent images show RAX-, OTX2-, MITF-D–, and RPE65-mCherry reporter expression in stably transduced UCSF4 hESCs during directed differentiation. RAX- and OTX2-mCherry reporters are expressed earliest in differentiation (days 4–6). MITF-D–mCherry reporter expression was detected later in directed differentiation (days 10–14). RPE65-mCherry reporter fluorescence was not detected during differentiation. Scale bar is 100 μm. (B) Quantitative real-time PCR analysis of endogenous RAX, OTX2, MITF-D, and RPE65 expression during directed differentiation is shown. Error bars represent ±SEM.
Date of download: 11/9/2017
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