1. MATERI 8 EXTRA EMBRYONIC GERM LAYER, IMPLANTASI DAN ORGANOGENESIS
MK. Ilmu Reproduksi
LABORATORIUM REPRODUKSI TERNAK FAPET UB

2. LABORATORIUM REPRODUKSI TERNAK FAPET UB
SUB POKOK BAHASAN
Implantasi
Pembelahan ectoderm, mesoderm dan endoderm
Material Recognition of embryo
Proses plasentasi dan lapisan-lapisan plasenta
Perkembangan embrio selama kebuntingan
Proses terbentuknya organ tubuh
Peristiwa apoptosis dalam pembentukan organ tubuh
LABORATORIUM REPRODUKSI TERNAK FAPET UB

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5. Implantation
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9. Proses Implantasi
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20. Development of Extraembryonic Membranes
Formation
of embryo
Ectoderm
Mesoderm
Outgrowth of
Hindgut
Endoderm
Four Placental Membranes
1. Chorion 2. Yolk Sac
3. Amnion Allantois
Day 16

21. Amnioinc
Cavity
Amnion
Chorion
Yolk Sac
Expands to
fill chorion
Allantoic Cavity
Day 18
Allantois
Chorio Allantois
Blood vessels
formed are connected to
fetus through umbilical cord

22. Function of Fetal Membranes and Fluids
Yolk sac: Early nutrient supply for early embryo.
Amnion: Protects fetus from injury, provides lubrication for
parturition, reservoir for urine and wastes.
Allantois: Fuses with chorion (chorio-allantoic placenta),
carries blood vessels of placenta, reservoir for
nutrients and wastes. Umbilical cord attaches fetus
to allantois.
Chorion: Attaches to uterus, absorbs nutrients from uterus,
allows maternal/fetal gas exchange, produces
hormones.
Picks-up uterine secretions from endometrium
to stimulate early embryonic development
Prevents the lungs from collapsing and opens
digestive tract
Fills with fluid to expand the Chorio-allantois
against the uterine wall

23. Attachment of the placenta to the uterus. (Implantation)
COW SOW EWE MARE
Days
Types of Implantation.
A. Superficial - Chorion fuses to endometrium
B. Interstitial - Embryo invades endometrium and develops in
the endometrium.

24. Implantation Window Decrease in Muc-1 on uterine epithelial surface
after progesterone receptor down-regulates
allows interaction of integrins to initiate
placental attachment allowing expansion of
trophoblast.
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25. Placental Attachment in Sow
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26. Maternal Recognition of Pregnancy Definition:
Establishment of Pregnancy Sow Mare Cow Ewe
Days of Estrous Cycle
Embryonic Signal
Chemical message (Usually hormonal) which results
in maintenance of the CL. Either through inhibiting
PGF2a production from the the endometrium,
preventing release from uterus or protecting CL
from PGF2a effects.
12, 15-18
14-16
16-17
12-13
Estrogen
Small
unknown
Peptide
Bovine Ovine
Interferon-t
In pigs, problem with moldy corn -
Aflotoxins are estrogenic (Ralgro)
Causes pseudopregnancy & early
embryonic death
Treatment: get off moldy corn and give PGF2a

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28. Cow, Ewe Only needs to Mare Embryo must be on side with
Capsule
Day 15
Embryo
Day 13
Picture provided
by Michel Guillomot
Cow, Ewe
Only needs to
be on side with
CL. No effect of
opposite horn
Mare
Embryo must
migrate between
horns from Days
Does it
almost 13X/Day
Pregnancy test in
Ruminants:
Protein B found
in blood by day 25
of Cow’s gestation
Endometrial cysts can cause problem
Treatment : If embryo cannot migrate
give progesterone until Day 150

29. Cotyledonary Placenta
Types of Placenta
Diffuse Placenta
Discoid Placenta
Chorionic
Plate
Mare
Sow
Cotyledonary Placenta
Human
Ewe
Cow
Also Goat & Deer

30. Placentome in Ruminants
Cow
Ewe
Chorion
Chorion
Caruncle
Caruncle
Endometrium
Endometrium
Mare, Sow
Chorion
Microcotyledon
Endometrium

31. Placental Attachment in Ruminants
Carry Placental
Lactogen into
maternal blood
Chorion
Binucleate
Cell
Migrate and
fuse with
uterine
epithelium
Uterine
Epithelium
Syncytium
Capillary
Fusion of
Binucleate
cells and
uterine
epithelium
(multinucleate)
Stroma
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32. Placentomes in Ruminants
Attachment of placental
cotyledons to caruncle
forms placentome
Maternal vascularity supplies
the needed nutrients for fetal
development

33. Placental-Uterine Attachment
Microcotyledon
(Fetal)
Umbilical Vessels
Allantois
Increase placental
surface area
Chorion
Endometrial
Epithelium
Micro-
cotyledon
(Maternal)
Micro-
cotyledon
Endometrial
Glands
Uterine
Arteries
Uterine
Veins
Redrawn with permission
Steven, D.H.& Samuel, C.A J. Reprod. Fertil. 23:579
Endometrium

34. Fetal Nutrition (Farm Animals)
Blood gas exchange at chorion - endometrium attachment.
Placenta highly permeable to water and electrolytes i.e., stored in
and exchange between allantoic and amniotic fluids.
Placenta converts glucose to fructose.
Fetal fat formed from fatty acids transferred across the placenta.
Fetus makes proteins from transferred amino acids.
Water soluble vitamins cross the placenta easier than fat soluble.
Minerals are transferred across the placenta.
Antibodies to fight disease do not cross the placenta - the
newborn will get these antibodies from the first milk - Colostrum.
Allows storage in allantoic
fluid
In Domestic Species

35. Placental Development in the Mare
Day 25
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36. Placental Development in the Mare
Day 34
Day 40
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38. Terimakasih
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