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4 Activities of SOD (A) and CAT (B) of P

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1 4 Activities of SOD (A) and CAT (B) of P
4 Activities of SOD (A) and CAT (B) of P. involutus grown in liquid MMN medium without Cd (X) or treated with 5 (▲), 50 (○), or 500 (●) μM Cd for 96 h. The activity was expressed relative to the activity in controls (=100%, dashed line). Each value is the mean of four individual replicates (±S.D.). * indicates values that differ significantly from the control at P≤0.05. From: Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium FEMS Microbiol Ecol. 2002;42(3): doi: /j tb01025.x FEMS Microbiol Ecol | © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

2 3 Antioxidative systems in P. involutus
3 Antioxidative systems in P. involutus. Fungal material of controls was collected after 3, 6, 24, 48 and 96 h culture time (n=4 per sampling date) and used to determine antioxidative systems. The bars indicate overall means from all sampling dates (n=24, ±S.D.). CATs, MDAR, DAR, GR, GPx, APX activities in nkat (mg protein)<sup>−1</sup> and SODs in U (mg protein)<sup>−1</sup>. Ascorbate (ASC) and glutathione (reduced and oxidised glutathione) (GSH) were calculated in nmol g<sup>−1</sup> fresh weight. Gray bars, left y axis; black bar, right y axis. ND, not detected. From: Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium FEMS Microbiol Ecol. 2002;42(3): doi: /j tb01025.x FEMS Microbiol Ecol | © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

3 2 Cd concentration in mycelia of P
2 Cd concentration in mycelia of P. involutus grown in liquid MMN medium treated with 5 (▲), 50 (○), or 500 (●) μM Cd for 96 h. The detection limit for cadmium determination was 30 ng mg<sup>−1</sup> dry weight indicated by the dashed line. * indicates values that differ significantly from the control at P≤0.05. From: Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium FEMS Microbiol Ecol. 2002;42(3): doi: /j tb01025.x FEMS Microbiol Ecol | © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

4 1 Biomass (A) and ergosterol content (B) of P
1 Biomass (A) and ergosterol content (B) of P. involutus grown in liquid MMN medium without Cd (X) or treated with 5 (▲), 50 (○), or 500 (●) μM Cd for 96 h. Each value is the mean of four individual replicates (±S.D.). * indicates values that differ significantly from controls at P≤0.05. From: Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium FEMS Microbiol Ecol. 2002;42(3): doi: /j tb01025.x FEMS Microbiol Ecol | © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

5 5 Activities of GPx (A) and GR (B) and concentration of glutathione (reduced and oxidised glutathione) (C) in P. involutus grown in liquid MMN medium without Cd (X) or treated with 5 (▲), 50 (○), or 500 (●) μM Cd for 96 h. The enzyme activity and the glutathione concentration were expressed relatively to controls (=100%, dashed line). Each value is the mean of four individual replicates (±S.D.). * indicates values that differ significantly from the control at P≤0.05. From: Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium FEMS Microbiol Ecol. 2002;42(3): doi: /j tb01025.x FEMS Microbiol Ecol | © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

6 6 Sections of P. involutus (1 μm thickness) embedded in styrene–methacrylate and photographed under a transmission electron microscope (Philips EM 420) with a magnification of 4900-fold. Bar=400 μm. From: Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium FEMS Microbiol Ecol. 2002;42(3): doi: /j tb01025.x FEMS Microbiol Ecol | © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

7 7 Correlation between sulphur and cadmium in ‘vacuolar bodies’ (see Fig. 6) in P. involutus grown in liquid MMN medium treated with 500 μM Cd for 96 h. From: Characterisation of antioxidative systems in the ectomycorrhiza-building basidiomycete Paxillus involutus (Bartsch) Fr. and its reaction to cadmium FEMS Microbiol Ecol. 2002;42(3): doi: /j tb01025.x FEMS Microbiol Ecol | © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.


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