1. Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA.
RNAs from the relevant wild type Col were isolated and converted to cDNAs. RT-PCR was done
with the primers as described in methods. Lanes in the gel from left to right: L: ladder; genomic Col
DNA as positive control, 1683 bp fragment: cDNA from Col as positive control, 1107 bp fragment;
AtplaIVA-1 cDNA, no fragment; AtplaIVA-2 cDNA, no fragment; cDNA Col, positive control for actin
expression, 1120 fragment; AtplaIVA-1 cDNA, positive control for actin expression, 1120 fragment;
AtplaIVA-2 cDNA, positive control for actin expression, 1120 fragment.
(B) AtplaIVB-1 and AtplaIVB-2 null transcription lines for AtPLAIVB mRNA. RNAs from the relevant
wild type Col were isolated and converted to cDNAs. RT-PCR was done with the primers as described i
n methods. Lanes in the gel from left to right: L: ladder; genomic Col DNA as positive control,
1737 bp fragment: cDNA from Col as positive control, 1035 bp fragment; AtplaIVB-1 cDNA, no
fragment; AtplaIVB-2 cDNA, no fragment; cDNA Col, positive control for actin expression, 1120
fragment; AtplaIVB-1 cDNA, positive control for actin expression, 1120 fragment; AtplaIVB-2 cDNA,
positive control for actin expression, 1120 fragment.
(C) AtplaIVC-1 null transcription line for AtPLAIVC mRNA. RNAs from the relevant wild type Ws were
isolated and converted to cDNAs. RT-PCR was done with the primers as described in methods.
Lanes in the gel from left to right: L: ladder; genomic Ws DNA as positive control; cDNA from Ws as
positive control; AtplaIVC-1 cDNA, no fragment; cDNA Ws, positive control for actin expression, 1120
fragment; AtplaIVC-1 cDNA, positive control for actin expression, 1120 fragment.