1. Effect of Gasoline on Coral Bleaching
Group 4:
Joon Chung
Jimmy Wesman
Nauman Chandry
Heather Hollembeak
Sebastian Camacho
Will Quartell

2. Question:
How does gasoline concentration affect the symbiosis between the polyps and zooxanthellae?
At what levels of gasoline concentration will polyps bleach?

3. Hypothesis
Due to 87 Octane acting as an anthropogenic factor, zooxanthallae will die off and coral bleaching will occur.
This will result in withering and ultimately, death.

4. Why Gasoline?
Outside stressors, such as gasoline spill, cause zooxanthallae to fall off by hindering the light from reaching it.
Oil seepage may poison the coral.

5. Our Variable: 87 Octane Week 1- 0.64 ml of gasoline in a tank
This is the closest variable we could get to regular gasoline oil.
Week ml of gasoline in a tank
According to Canadian Environmental Assessment Agency, standard is 80 micrograms per gram of water in Canada. This converts to 0.64 ml / tank (80L).
Week ml
We will increase the amount of gasoline by 0.64.

6. Procedures
Regular Grade (87 octane) gasoline will be poured at a specific concentration, 0.64 ml, every week.
zooxanthallae counts, chlorophyll concentrations, and protein concentration will be recorded every week.
Analyze Results
Draw conclusions based on growth/death rates of Coral in tank

8. The End