1. Laboratory
Safety Rules

2. While working in the science laboratory, you will have certain important _____________ that do not apply to other classrooms. You will be working with materials and apparatus which, if handled carelessly or improperly, have the potential to cause ______ or discomfort to someone else as well as yourself.
responsibilities
injury

3. A science laboratory can be a safe place in which to work if you, the student, are foresighted, alert, and cautious. Violating any of the following regulations will result in you being suspended from class or permanently removed from the class. The following practices will be followed:

4. instructor
1. An _________ must be present during the performance of all laboratory work.
2. Report any _________ to the teacher immediately, no matter how_______, including reporting any burn, scratch, cut, or corrosive liquid on skin or clothing.
3. Prepare for each laboratory activity by _______ all instructions before coming to class. Follow all _________ implicitly and intelligently. Make note of any __________ in procedure given by the instructor.
accident
minor
reading
directions
modification

5. spill
4. Any science project or individually planned experiment must be __________ by the teacher.
approved
5. Use only those materials and equipment
_________ by the instructor.
authorized
6. Inform the teacher___________ of any
equipment not working properly.
immediately
7. Clean up any nonhazardous _____ on
the floor or workspace __________.
spill
immediately

6. Anyone caught not wearing their goggles (teacher included) must sing a goggle song for the whole class. If you feel this is too embarrassing, have the whole class sing the song anytime one of them is caught. Here's an example of a song sung to the tune of "I'm a Little Teapot."
I'm a little Chemist, short and stout,
Here are my goggles, here are my eyes.
When I don't wear my goggles, my teacher shouts,
PUT THEM ON OR YOU GET OUT!

7. Wear Your Safety Goggles! Which would you rather read?
FLINN Scientific, Inc "Your Safer Source for Science Supplies" - Poster

8. I'm a little Chemist, short and stout,
Here are my goggles, here are my eyes.
When I don't wear my goggles,
my teacher shouts,
PUT THEM ON OR YOU GET OUT!
Anyone caught not wearing their goggles (teacher included) must sing a goggle song for the whole class. If you feel this is too embarrassing, have the whole class sing the song anytime one of them is caught. Here's an example of a song sung to the tune of "I'm a Little Teapot."
I'm a little Chemist, short and stout,
Here are my goggles, here are my eyes.
When I don't wear my goggles, my teacher shouts,
PUT THEM ON OR YOU GET OUT!

9. eye protection
8. Wear appropriate ______________, as directed by the instructor, whenever you are working in the laboratory. Safety goggles must be worn during hazardous _________ involving caustic/corrosive chemicals, heating of liquids, and other activities that may injure the eyes.
activities

10. 9. Splashes and fumes from hazardous chemicals present a special danger to wearers of _____________. Therefore, students should preferably wear regular glasses (inside splash -proof goggles, when appropriate) during all class activities or purchase personal splash-proof goggles and wear them whenever exposure to chemicals or chemical fumes is possible. Students with open skin wounds on hands must wear gloves or be excused from the laboratory activity.
contact lenses

11. 10. Never ______ hot equipment or dangerous chemicals through a ______ of students.
11. Check ______ and equipment instructions carefully. Be sure correct items are ______ in the proper manner.
12. Be aware if the _________ being used are hazardous. Know where the material safety data sheet (______) is and what it indicates for each of the hazardous chemicals you are using.
carry
group
labels
used
chemicals
MSDS

12. taste
13. Never ______ anything or touch chemicals with the hands, unless __________ instructed to do so.
specifically
14. Test for odor of chemicals only by ______ your hand above the container and sniffing cautiously from a ________.
waving
distance
15. Eating or drinking ___ the laboratory or from laboratory equipment is ____ permitted. in
NOT
16. Use a __________ pipette filler (never the mouth) when measuring or transferring small quantities of liquid with a pipette.
mechanical

13. 17. When heating material in a test tube, do not _____ into the tube or point it in the direction of any person during the process.
look
18. Never pour ________ back into bottles, exchange stoppers of bottles, or lay stoppers on the table.
reagents
19. When diluting _____, always pour acids into ______, never the reverse. Combine the liquids slowly while stirring to distribute heat buildup throughout the mixture.
acids
water

14. 20. Keep _______ away from face, eyes, and clothes while using solutions, specimens, equipment, or materials in the laboratory. Wash hands as necessary and wash thoroughly at the __________ of the laboratory period.
hands
conclusion
21. To treat a burn from an acid or alkali, wash the affected area ___________ with plenty of running water. If the eye is involved, irrigate it at the eyewash station without interruption for 15 minutes. Report the incident to your instructor immediately.
immediately

15. 22. Know the ________ of the emergency shower and eyewash station, fire blanket, fire extinguisher, fire alarm box, and exits.
23. Know the proper fire and earthquake drill ___________.
24. Roll long sleeves above the _____. Long, hanging necklaces, bulky jewelry, and excessive and bulky clothing should not be worn in the laboratory.
25. Confine long hair during a _________ activity.
location
procedures
wrist
laboratory

16. cover
26. Wear shoes that _____ the toes, rather than sandals, in the laboratory.
clean
27. Keep work areas _____. Floors and aisles should be kept clear of equipment and materials.
burners
28. Light gas ________ only as instructed by the teacher. Be sure no _______ materials (such as alcohol or acetone) are being used nearby. Use a burner with ________ caution. Keep your head and clothing away from the flame and turn it off when not in use.
volatile
extreme

17. 29. Use a fire blanket (stop, drop, and roll) to _________ any flame on a person.
extinguish
30. Dispose of laboratory waste as _________ by the teacher. Use separate, designated containers (not the ___________) for the following:
instructed
wastebasket
Matches, litmus paper, wooden splints, __________, and so on
toothpicks
Broken
_______ and waste glass
Rags, paper towels, or other absorbent materials used in the cleanup of __________ solids or liquids
flammable
liquids
Hazardous/toxic ______ and solids

18. 31. Place books, purses, and such items in the designated storage area
31. Place books, purses, and such items in the designated storage area. Take only _________ manuals and notebooks into the _______ area.
laboratory
working
32. Students are not permitted in laboratory _______ rooms or teachers' workrooms without the approval of the ________
storage
teacher.
33. To cut small diameter glass tubing, use a ____ or tubing cutter to make a deep scratch. Wrap the tubing in a paper towel before breaking the glass away from you with your thumbs. Fire polish all ends.
file

19. 34. Hot and cold glass has the same _____ appearance
34. Hot and cold glass has the same _____ appearance. Determine whether an object is hot by bringing the back of your hand close to the object.
visual
35. Match hole sizes and tubing when inserting glass tubing into a stopper. If necessary, expand the hole first by using an appropriate size cork borer. Lubricate the stopper hole and glass tubing with water or glycerin to ease insertion, using ______ to protect the hand. Carefully twist (never push) glass tubing into stopper holes.
towels

20. broken
36. Remove all ________ glass from the work area or floor as soon as possible. Never handle broken glass with bare hands; use a counter _____ and dustpan.
brush
37. Report broken glassware, including thermometers, to the instructor __________.
immediately.
38. Operate electrical equipment only in a dry area and with _____ hands.
dry
39. When removing an electrical plug from its socket, pull the ______, not the electrical cord.
plug

21. 40. Always approach laboratory experiences in a _______ and courteous manner.
serious
clean
41. Always ______ the laboratory area before leaving.
42. Students and teacher wash hands with ______ and water before leaving the laboratory area.
soap

22. 43. When heating volatile or flammable materials, use a water bath; that is, heat the materials in or over heated water, using a hot plate to heat the water. _________ all open flames.
Extinguish
caution
44. Exercise _______ in using scissors, scalpels, dissecting needles, and other sharp-edged instruments. Pass them with handles extended when handing them to other persons.

23. immediately
45. Inform the teacher ___________ of any equipment not working properly.
46. Use the fume hood whenever noxious, corrosive, or toxic _______ are produced or released.
fumes
clean
47. Be sure all glassware is ______ before use. Clean glassware thoroughly ______ use. Residue may cause errors in new experiments or cause a _______ reaction or explosion.
after
violent

24. The following actions will result in being dropped from the class:
Misuse
_______ of safety equipment including, but not limited to
eyewash stations
emergency showers
fire extinguishers
fire blanket
Vandalizing
__________ school equipment or furniture.
Endangering
__________ or causing harm to others.

26. Chemical Hazard Label MSDS Safety Quiz
LABORATORY SAFETY
Chemical Hazard Label
MSDS
Safety Quiz
Courtesy Christy Johannesson

27. Safety Symbols

28. NFPA CHEMICAL HAZARD LABEL
FLAMMABILITY
HEALTH
REACTIVITY
(STABILITY)
SPECIAL

29. NFPA CHEMICAL HAZARD LABEL
Least
Serious 4
Most 4 2 3 4
Flammable vapor which burns readily
Substance is stable

30. NFPA CHEMICAL HAZARD LABEL
Burns readily.
Diborane 4 3 W
May detonate with heat or ignition.
Severe health risk.
Avoid water.

31. NFPA CHEMICAL HAZARD LABEL
Complete Label for Acetone

32. NFPA CHEMICAL HAZARD LABEL
Complete Label for Phosphine

33. MSDS Material Safety Data Sheet On file for all purchased chemicals.
Includes all information shown on a chemical label and more.
Different formats are used by different chemical companies.

34. MSDS

35. Health Hazard 4
Very short exposure could cause death or serious residual injury even though prompt medical attention was given. 3
Short exposure could cause serious temporary or residual injury even though prompt medical attention was given. 2
Intense or continued exposure could cause temporary incapacitation or possible residual injury unless prompt medical attention is given. 1
Exposure could cause irritation but only minor residual injury even if no treatment is given.
Exposure under fire conditions would offer no hazard beyond that of ordinary combustible materials.

36. Must be preheated before ignition can occur.
FLAMMABILITY 4
Will rapidly or completely vaporize at normal pressure and temperature, or is readily dispersed in air and will burn readily. 3
Liquids and solids that can be ignited under almost all ambient conditions. 2
Must be moderately heated or exposed to relatively high temperature before ignition can occur. 1
Must be preheated before ignition can occur.
Materials that will not burn.

37. 1 Prior to 1996, this section was titled "Reactivity"
1 Prior to 1996, this section was titled "Reactivity". The name was changed because many people did not understand the distinction
between a "reactive hazard" and the "chemical reactivity" of the material. The numeric ratings and their meanings remain unchanged.
INSTABILITY1 4
Readily capable of detonation or of explosive decomposition or reaction at normal temperatures and pressures. 3
Capable of detonation or explosive reaction, but requires a strong initiating source or must be heated under confinement before initiation, or reacts explosively with water. 2
Normally unstable and readily undergo violent decomposition but do not detonate. Also: may react violently with water or may form potentially explosive mixtures with water. 1
Normally stable, but can become unstable at elevated temperatures and pressures or may react with water with some release of energy, but not violently.
Normally stable, even under fire exposure conditions, and are not reactive with water.

38. OX
This denotes an oxidizer, a chemical which can greatly increase the rate of combustion/fire.
          
Unusual reactivity with water. This indicates a potential hazard using water to fight a fire involving this material.
This section is used to denote special hazards. There are only two NFPA 704 approved symbols

39. ACID
This indicates that the material is an acid, a corrosive material that has a pH lower than 7.0
ALK
This denotes an alkaline material, also called a base. These caustic materials have a pH greater than 7.0
                        
This denotes a material that is corrosive (it could be either an acid or a base).
The skull and crossbones is used to denote a poison or highly toxic material. See also: CHIP Danger symbols.
The international symbol for radioactivity is used to denote radioactive hazards; radioactive materials are extremely hazardous when inhaled.
Indicates an explosive material. This symbol is somewhat redundant because explosives are easily recognized by their Instability Rating.