1. BCA
blue complex

2. BCA assay Procedure summary： Plot standard curve Convert sample value
PierceReagent A：Reagent B = 50：1
Procedure summary：
Plot standard curve
Convert sample value
10 ul sample + 190u l Working reagent

3. Standard curve

4. SCIENCE IMAGING SYSTEMS
-- Introduction to LAS-3000

5. Are You X-film generation ?
You need to……
Waste time
Waste x-film
Production more waste liquid
Living in the dark

6. Are You X-film generation ?
You need to……
Scanner
Density meter
Analysis software

7. Are You Ready to Change ? You just need to …… Living in the light !!
LAS-3000
A hand
A smile

8. What Can You do with LAS-3000
Chemiluminescence
ECL
ECL Plus
Super Signal
CDP-Star
Fluorescence
Ethidium bromide (EtBr)
SYBR® Green I
AttoPhos,DDAO Phosphate
Digitize
Coomassie Brilliant Blue (CBB)
Sliver stain
X-ray

9. Inside of Intelligent dark box (IDX)
3.2M M pixel
CCD
F0.85
F2.8
White
Blue(460 nm)
Green(520 nm)
Red(630nm)
UV(312nm)
White

10. Carefully designed cooled CCD system equals the sensitivity of film
X-ray film
(3 min. exposure)
10pg 1pg 500fg 200fg 100fg 50fg 20fg 10fg 5fg TE
LAS-1000
(2 min. exposure)
LAS has comparable sensitivity to X-ray film

11. Successful sample of LAS-3000 ！！
JOURNAL OF VIROLOGY, Dec. 2003, p –13072

14. Multi Gauge 3.0
Get A more accurate data……