1. Fig. 2. Effect of PGE2 on VEGF production in NPDFs as determined by RT-PCR (A, C) and ELISA (B, D). (A, B) RT-PCR and ELISA showed that the relative ratio of VEGF/GADPH mRNA and VEGF protein were increased in a dose-dependent manner. (C, D) The results of RT-PCR and ELISA showed that the relative ratio of VEGF/GADPH mRNA and VEGF protein were increased in a time-dependent manner. Monolayer cultures were stimulated with PGE2 (10 mM), and the medium was harvested at varying time points followed by determination of VEGF levels by ELISA. The means±SE of data from six experiments are shown. *P<0.05 and †P<0.01 vs. control. VEGF, vascular endothelial growth factor; GAPDH, glyceraldehyde phosphate dehydrogenase; PGE2, prostaglandin E2; NPDF, nasal polyp derived fibroblast; RT-PCR, reverse transcription-polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; SE, standard error.
Fig. 2. Effect of PGE2 on VEGF production in NPDFs as determined by RT-PCR (A, C) and ELISA (B, D). (A, B) RT-PCR and ELISA showed that the relative ratio of VEGF/GADPH mRNA and VEGF protein were increased in a dose-dependent manner. (C, D) The results of RT-PCR and ELISA showed that the relative ratio of VEGF/GADPH mRNA and VEGF protein were . . .
Allergy Asthma Immunol Res Jul;5(4):