1. BME 273 Senior Design Project Group 25

2. BioMEMs cell culture device
Scientific Design
BioMEMs cell culture device

3. Cell Culture Design

4. Cross Section

5. Materials Polydimethlysiloxane (PDMS) Negative Resist (SU-8)
Silicon Wafers
MEMS laboratory
8 mm masks
Platinum (working electrodes)
Silver (reference Ag/AgCl electrodes)

6. Fabrication Steps
Lay down SU-8 on silicon wafer, expose using mask, and develop lower region for cell insertion and perfusion.
Cast PDMS replica of master
Lay down SU-8 on silicon wafer, expose using mask, and develop upper region for pneumatic control of cell insertion channels.
Cast PDMS replica of master and then lay over top of lower region

7. Developing a strategy to present to venture capitalists
Business Strategy
Developing a strategy to present to venture capitalists

8. Components Market & Demand Environment Technological Environment
Competitive Environment
Economic & Policy Environment
Strategic Implications of Innovation Success Factors
Technology Intelligence (Contacts)
Product Development
Financing

9. Main Focus Points Nano-scale
Simulate in vivo better than current methods
Scale up the number of cell cultures per experiment
Automated experiments
Cost efficiency
Portable experimentation?

10. Applications Replace conventional cell culture techniques
Scale up
Reduce number of lab technicians  cost efficient
Drug development
Monitor cells before & after drug

11. Drug Companies Pfizer Johnson & Johnson GlaxoSmithKline Novartis Merck
Eli Lilly
Abbott Laboratories

12. Project Schedule – Microsoft Project

13. Project Schedule – Gant Chart

14. References http://finance.yahoo.com
Fabrication of miniature Clark oxygen sensor integrated with microstructure
Ching-Chou Wu, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
A BioMEMS Review: MEMS Technology for Physiologically Integrated Devices
AMY C. RICHARDS GRAYSON, REBECCA S. SHAWGO, AUDREY M. JOHNSON, NOLAN T. FLYNN, YAWEN LI, MICHAEL J. CIMA, AND ROBERT LANGER