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Experimental Hematology

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Presentation on theme: "Experimental Hematology"— Presentation transcript:

1 Experimental Hematology
Human bone marrow mesenchymal stem cells secrete endocannabinoids that stimulate in vitro hematopoietic stem cell migration effectively comparable to beta adrenergic stimulation  Sevil Köse, Fatima Aerts-Kaya, ağla Zübeyde Köprü, Emirhan Nemutlu, Barış Kuşkonmaz, Beren Karaosmanoğlu, Zihni Ekim Taşkıran, Belgin Altun, Duygu Uçkan Çetinkaya, Petek Korkusuz  Experimental Hematology  DOI: /j.exphem Copyright © Terms and Conditions

2 Figure 1 Characterization of human BM-MSCs. Stromal and multipotential nature of MSCs were morphologically, immune-phenotypically and through differentiation confirmed. (A) Representative histograms showing specific markers for MSCs by FC. FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes. (B) Bar graphic showing the percentage of negative (CD34, CD45) and positively (CD73, CD44, CD90, CD29) labeled MSCs in G-CSF untreated and treated groups (n=10 for each group). (C) Oil Red O (ORO) staining for adipogenic cells (inverted microscope, Olympus, 10X) and (D) Alizarin Red S (ARS) for osteogenic cells (inverted microscope, Olympus, 10X) are positive after 21 days of differentiation in appropriate media (n=10 for each group). (E) The amount of Ca2+ and ORO dye for osteogenic and adipogenic differentiation of MSCs respectively (n=10 for each group) was semi-quantitatively measured. Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

3 Figure 1 Characterization of human BM-MSCs. Stromal and multipotential nature of MSCs were morphologically, immune-phenotypically and through differentiation confirmed. (A) Representative histograms showing specific markers for MSCs by FC. FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes. (B) Bar graphic showing the percentage of negative (CD34, CD45) and positively (CD73, CD44, CD90, CD29) labeled MSCs in G-CSF untreated and treated groups (n=10 for each group). (C) Oil Red O (ORO) staining for adipogenic cells (inverted microscope, Olympus, 10X) and (D) Alizarin Red S (ARS) for osteogenic cells (inverted microscope, Olympus, 10X) are positive after 21 days of differentiation in appropriate media (n=10 for each group). (E) The amount of Ca2+ and ORO dye for osteogenic and adipogenic differentiation of MSCs respectively (n=10 for each group) was semi-quantitatively measured. Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

4 Figure 2 BM-MNCs and CD34+HSCs, express CB1, CB2 and various subtypes, by FC and qRT-PCR. (A) Representative histograms showing specific markers for MNCs obtained after density gradient centrifugation method. Monocyte/lymphocyte/stem cell fraction (as gated in P1) of the MNCs were used for receptor analysis (n=10 for each group). (B) Representative histograms showing specific markers for CD34+HSCs (Q1+Q2 quadrants on first graphic) by FC. (n=10 for each group). (C) Representative histograms showing specific markers for BM MSCs by FC. MSCs were gated according to their own FSC/SSC plot (n=10 for each group). (D) Bar graphic showing the percentage of MNC, MSC and CD34+HSCs expressing Adrβ1, 2, 3 and CB1, CB2 receptors are shown by FC. The MNCs and CD34+HSCs express significantly higher levels of CB1 and CB2 receptors in G-CSF untreated and treated groups, when compared to MSCs. CD34+HSCs exhibit higher expression for Adrβ2 and Adrβ3 in all groups comparing to MNCs; but the difference is only significant for Adrβ3 (n=10 for each group). (E, F) qRT-PCR analysis reveals CB1 and CB2 gene expressions on MNCs, but not on MSCs. MNCs and MSCs mainly express Adrβ2 (n=6 for each group). FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes (#) p<0,05 Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

5 Figure 2 BM-MNCs and CD34+HSCs, express CB1, CB2 and various subtypes, by FC and qRT-PCR. (A) Representative histograms showing specific markers for MNCs obtained after density gradient centrifugation method. Monocyte/lymphocyte/stem cell fraction (as gated in P1) of the MNCs were used for receptor analysis (n=10 for each group). (B) Representative histograms showing specific markers for CD34+HSCs (Q1+Q2 quadrants on first graphic) by FC. (n=10 for each group). (C) Representative histograms showing specific markers for BM MSCs by FC. MSCs were gated according to their own FSC/SSC plot (n=10 for each group). (D) Bar graphic showing the percentage of MNC, MSC and CD34+HSCs expressing Adrβ1, 2, 3 and CB1, CB2 receptors are shown by FC. The MNCs and CD34+HSCs express significantly higher levels of CB1 and CB2 receptors in G-CSF untreated and treated groups, when compared to MSCs. CD34+HSCs exhibit higher expression for Adrβ2 and Adrβ3 in all groups comparing to MNCs; but the difference is only significant for Adrβ3 (n=10 for each group). (E, F) qRT-PCR analysis reveals CB1 and CB2 gene expressions on MNCs, but not on MSCs. MNCs and MSCs mainly express Adrβ2 (n=6 for each group). FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes (#) p<0,05 Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

6 Figure 3 AEA and 2-AG stimulate CD34+ PBSC migration to MSCs and this migration effect is blocked by Adrβ and CB receptor antagonists. (A) Representative histograms showing isotype controls and specific markers for CD34+ PBSCs (Q1+Q2 quadrants on fourth graphic) by FC (n=6 for each group). (B) Characterization of PBSCs and CD34+ cells from apheresis product for the β-AR and CB receptors by FC (n=6). The cells expressed Adrβ subtypes and the CB receptors. (C) Experimental design for transwell migration assay is shown. The transwell/co-culture system allows CD34+ PBSC migration toward SDF-1, norepinephrine, AEA, 2-AG or MSCs respectively. (D) The CD34+ PBSCs migration toward SDF-1 and NE are inhibited by specific antagonists AMD3100 and SR59230A respectively (n=6). (E) Migration of CD34+ PBSCs to eCBs; AEA and 2-AG. CD34+ PBSCs exhibited significantly higher migration to 30nM and 50µM doses of AEA, and 30nM, 1µM and 50µM doses of 2-AG, when compared to SDF-1. This migration effect is blocked by CB antagonists (* p<0.05, n=6). (F) CD34+ PBSCs effectively migrated towards LPS stimulated (LPS+) and unstimulated (LPS-) MSCs. The LPS-only control group is not presented since its at the base line. Migration effect is blocked by CB1 antagonist AM281, CXCR4 antagonist AMD3100 and the Adrβ blocker SR59230A significantly (* p<0.05, n=6). FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes. Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

7 Figure 3 AEA and 2-AG stimulate CD34+ PBSC migration to MSCs and this migration effect is blocked by Adrβ and CB receptor antagonists. (A) Representative histograms showing isotype controls and specific markers for CD34+ PBSCs (Q1+Q2 quadrants on fourth graphic) by FC (n=6 for each group). (B) Characterization of PBSCs and CD34+ cells from apheresis product for the β-AR and CB receptors by FC (n=6). The cells expressed Adrβ subtypes and the CB receptors. (C) Experimental design for transwell migration assay is shown. The transwell/co-culture system allows CD34+ PBSC migration toward SDF-1, norepinephrine, AEA, 2-AG or MSCs respectively. (D) The CD34+ PBSCs migration toward SDF-1 and NE are inhibited by specific antagonists AMD3100 and SR59230A respectively (n=6). (E) Migration of CD34+ PBSCs to eCBs; AEA and 2-AG. CD34+ PBSCs exhibited significantly higher migration to 30nM and 50µM doses of AEA, and 30nM, 1µM and 50µM doses of 2-AG, when compared to SDF-1. This migration effect is blocked by CB antagonists (* p<0.05, n=6). (F) CD34+ PBSCs effectively migrated towards LPS stimulated (LPS+) and unstimulated (LPS-) MSCs. The LPS-only control group is not presented since its at the base line. Migration effect is blocked by CB1 antagonist AM281, CXCR4 antagonist AMD3100 and the Adrβ blocker SR59230A significantly (* p<0.05, n=6). FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes. Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

8 Figure 3 AEA and 2-AG stimulate CD34+ PBSC migration to MSCs and this migration effect is blocked by Adrβ and CB receptor antagonists. (A) Representative histograms showing isotype controls and specific markers for CD34+ PBSCs (Q1+Q2 quadrants on fourth graphic) by FC (n=6 for each group). (B) Characterization of PBSCs and CD34+ cells from apheresis product for the β-AR and CB receptors by FC (n=6). The cells expressed Adrβ subtypes and the CB receptors. (C) Experimental design for transwell migration assay is shown. The transwell/co-culture system allows CD34+ PBSC migration toward SDF-1, norepinephrine, AEA, 2-AG or MSCs respectively. (D) The CD34+ PBSCs migration toward SDF-1 and NE are inhibited by specific antagonists AMD3100 and SR59230A respectively (n=6). (E) Migration of CD34+ PBSCs to eCBs; AEA and 2-AG. CD34+ PBSCs exhibited significantly higher migration to 30nM and 50µM doses of AEA, and 30nM, 1µM and 50µM doses of 2-AG, when compared to SDF-1. This migration effect is blocked by CB antagonists (* p<0.05, n=6). (F) CD34+ PBSCs effectively migrated towards LPS stimulated (LPS+) and unstimulated (LPS-) MSCs. The LPS-only control group is not presented since its at the base line. Migration effect is blocked by CB1 antagonist AM281, CXCR4 antagonist AMD3100 and the Adrβ blocker SR59230A significantly (* p<0.05, n=6). FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes. Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

9 Figure 3 AEA and 2-AG stimulate CD34+ PBSC migration to MSCs and this migration effect is blocked by Adrβ and CB receptor antagonists. (A) Representative histograms showing isotype controls and specific markers for CD34+ PBSCs (Q1+Q2 quadrants on fourth graphic) by FC (n=6 for each group). (B) Characterization of PBSCs and CD34+ cells from apheresis product for the β-AR and CB receptors by FC (n=6). The cells expressed Adrβ subtypes and the CB receptors. (C) Experimental design for transwell migration assay is shown. The transwell/co-culture system allows CD34+ PBSC migration toward SDF-1, norepinephrine, AEA, 2-AG or MSCs respectively. (D) The CD34+ PBSCs migration toward SDF-1 and NE are inhibited by specific antagonists AMD3100 and SR59230A respectively (n=6). (E) Migration of CD34+ PBSCs to eCBs; AEA and 2-AG. CD34+ PBSCs exhibited significantly higher migration to 30nM and 50µM doses of AEA, and 30nM, 1µM and 50µM doses of 2-AG, when compared to SDF-1. This migration effect is blocked by CB antagonists (* p<0.05, n=6). (F) CD34+ PBSCs effectively migrated towards LPS stimulated (LPS+) and unstimulated (LPS-) MSCs. The LPS-only control group is not presented since its at the base line. Migration effect is blocked by CB1 antagonist AM281, CXCR4 antagonist AMD3100 and the Adrβ blocker SR59230A significantly (* p<0.05, n=6). FITC: Fluoro-isothiocyanate, PE: Phycoerythrin, APC: Allophycocyanin and, PE-Cy7: PE-Cyanin 7 present conjugated antibodies or their isotypes. Experimental Hematology DOI: ( /j.exphem ) Copyright © Terms and Conditions

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