1. Pre-genomic era: finding your own clones

5. GENOME 1 2 3 23
CHROMOSOMES
YACs or
BACs (map)
Plasmid
Sub-clones

6. Gridded (arrayed/ordered) library

7. 18,000 BACs for Drosophila
300,000 BACs for Humans

8. STS- Sequence Tagged Site Mapping
Probe or
PCR product

11. Minimal Tiling Path

12. Sub-clone into smaller segments and map
Or use primer walking- NOT EFFICIENT
Plasmids
Or M13
Sequence nt
From each end
Primer walk

13. Sub-clone
End sequences

15. Virtual DNA
(sequence)
Assembly

17. Shotgun
Sequencing
2 or 3 libraries of
Different size fragments

18. Mates: read-pairs
2kb library of clones
10kb library of clones

19. * * * * * * * *

20. GENOME 1 2 3 23 YACs or BACs (map) Plasmid Sub-clones Don’t map
CHROMOSOMES
YACs or
BACs (map)
Plasmid
Sub-clones
Don’t map
Sequence nt
Assemble sequence of BAC

21. Mates: read-pairs 2kb library of clones 10kb library of clones

22. Cosmids, YACs ordered
C. Elegans 100Mb
Drosophila 120Mb
BACs ordered- STS mapping
+ fingerprinting
Whole Genome shotgun
Human 3,200Mb
BACs ordered- fingerprinting
Whole Genome shotgun