1. Dr Sunil Kumar Nanda, Bharathy N, Dr Lopamudra Ray
Title : Establishment of reference ranges for Lipid profile in puducherry
Dr Sunil Kumar Nanda, Bharathy N, Dr Lopamudra Ray

2. Background
IFCC recommends each laboratory to set its own reference ranges for the parameters.
In India, most of the laboratories use reference ranges from the kit literature of the manufacturers which are generally based on Western population.
The western reference ranges do not match with Indian population for many parameters like lipid profile

3. Since lipid profile values have direct relationship with development of coronary artery disease, it becomes much more important to follow a reference range which is applicable for the local population.
There are very few studies done to establish the reference ranges for lipid profile in Puducherry. Hence this study was taken up.

4. Aims and Objectives :
To establish reference ranges for Total Cholesterol, HDL Cholesterol, LDL Cholesterol and Triglycerides in Puducherry

5. Materials and methods
The study was a retrospective study fasting samples of healthy individuals between the age group of 20 to 70 years were analyzed for Total cholesterol, HDL cholesterol, LDL Cholesterol and Triglycerides.

6. Inclusion criteria
Healthy individuals who visited our hospital for master health check up were included in the study

7. Exclusion criteria 1. Subjects not fasting for 12 hrs
2. Subjects taking lipid lowering drugs
3. Diabetes Mellitus
4. Dyslipidemia
5. Smoking
6. Alcoholics
7.Pregnancy

8. Methods Total cholesterol : Cholesterol oxidase method
HDL Cholesterol : Direct HDL method
Triglycerides : Enzymatic colorimetric
LDL Cholesterol : Direct LDL method
The above mentioned parameters were measured in cobas integra 400 plus
We participated in Biorad eqas and CMC eqas

9. Results and observations
Sl No
Parameter
Mean + SD (mg/dl) 1
Total Cholesterol
190+47 2
HDL Cholesterol
41+10 3
LDL Cholesterol
137+73 4
Triglycerides
133+45

10. Discussion SL No Parameter Present study Kaur V et al (Punjab)
Das M et al
(Guwahati)
Median
Durgawale P et al
(Maharashtra) 1
T.Cholesterol
190+47
182.2 ± 33.9
170 (93-263)
165.7±30.2 2
HDL Cholesterol
41+10
44.1 ± 6.8
40 (23-73)
44.86±10.68 3
LDL Cholesterol
137+73
113.9 ± 32.0
103 (40-173)
101.66±29.8 4
Triglycerides
133+45
122.4 ± 33.4
110 (40-256)
88.36±31.2

11. Conclusion
Our results shows higher difference in reference ranges for LDL cholesterol when compared to LDL values of other regions of India
We recommend studies to be done on reference ranges of lipid profile with a larger sample size