1. Recall that our first case of thinking about pigments in the eye was to consider rhodopsin alone (one pigment) and the implications of its absorption spectrum. We had two lights, each with one wavelength. The left hand light was fixed and the right hand light could be varied in intensity. Could there be any intensity where the two lights look identical even though their wavelengths are different? Now add a pigment to the receptor system. Compare the response of two pigments to the two lights, each with a different wavelength from the other (one wavelength).

2. Case 1 -- Equal number of photons at each of the two different wavelengths

3. Two different wavelength lights indicated by Greek lambdas
Incident Photons
Pigment 1
Photons Absorbed
Pigment 2
Photons Absorbed
Incident
Pigment 1
Pigment 2

4. Case 2 -- Turn up the intensity of the adjustable light on the right
Case 2 -- Turn up the intensity of the adjustable light on the right. What happens to the number of photons absorbed by pigment 1 and pigment 2? Does either pigment match the corresponding number for the left hand light?
Remember: Once the wavelengths have been selected and the observer with a certain number of visual pigments has been selected, only the intensity (amount, number of photons) of the lights can be changed in the experiment.

5. Incident Photons
Pigment 1
Photons Absorbed
Pigment 2
Photons Absorbed
Incident
Pigment 1
Pigment 2
Photons Absorbed

6. Case 3 -- Turn down the intensity of the adjustable light on the right
Case 3 -- Turn down the intensity of the adjustable light on the right. What happens to the number of photons absorbed by pigment 1 and pigment 2? Does either pigment match the corresponding number for the left hand light?

7. Incident Photons
Pigment 1
Photons Absorbed
Pigment 2
Photons Absorbed
Incident
Pigment 1
Pigment 2

8. The two lights NEVER match

9. Summary Table What have we learned? 1 2 3 4 5 6 YN
As the wavelength intensity is adjusted, is there any place where the two lights exactly match? Yes, if we have one underlying pigment. No if we have 2 underlying pigments
Number of wavelengths in the adjustable light YN
Number of underlying pigments

10. Now add a second adjustable wavelength to the light on the right
Now add a second adjustable wavelength to the light on the right. Instead of one adjustable wavelength, we now have two adjustable wavelengths in an additive mix in the light on the right.

11. Spectral absorption curves for the two hypothetical pigments and two adjustable wavelengths in the light on the right (2 and 3 ) compared to the fixed light on the left (1)

12. Pigment 1
Pigment 2
Wavelengths in Lights

13. Person whose eye has two cone types (pigments)
Matching task: Can we adjust the amount of light in each light so that the lights look identical? If “yes,” then wavelength and intensity (amount of light) cannot be distinguished. If “no,” then wavelength and intensity can be distinguished. l1
l2 + l3
Set-up: Light with one
wavelength compared to
light with two wavelengths.
Eye
Person whose eye has two cone types (pigments)
Blow-up diagram of small portion of
retina – suppose there are two cone
types, meaning 2 pigments

14. l1 l2 + l3 Incident Photons Pigment 1 Photons Absorbed Pigment 2  
Total Effect

15. Incident Photons
Pigment 1
Photons Absorbed
Pigment 2
Photons Absorbed
Incident
Pigment 1
Pigment 2   
Total Effect

16. Now you got the two lights to match perfectly again
Now you got the two lights to match perfectly again. To do this you had to have two adjustable wavelengths being shined on two pigments

17. Summary Table 1 2 3 4 5 6 YN Number of underlying pigments Y
As the wavelength intensity is adjusted, is there any place where the two lights exactly match?
Number of wavelengths in the adjustable light YN
Number of underlying pigments Y

18. Now add a 3rd underlying pigment
What will happen when we try to match the light on the right, with two adjustable pigments, to the light on the left when there are 3 underlying responding pigments?

19. Pigment 1
Pigment 2
Draw a third
curve in here
Wavelengths in Lights

20. Person whose eye has three cone types (pigments)
Matching task: Can we adjust the amount of light in each light so that the lights look identical? If “yes,” then wavelength and intensity (amount of light) cannot be distinguished. If “no,” then wavelength and intensity can be distinguished. l1
l2 + l3
Set-up: Light with one
wavelength compared to
light with two wavelengths.
Eye
Person whose eye has three cone types (pigments)
Blow-up diagram of small portion of
retina – suppose there are three cone
types, meaning 3 pigments

21. No Match

22. Summary Table 1 2 3 4 5 6 Y Number of underlying pigments Y N
As the wavelength intensity is adjusted, is there any place where the two lights exactly match?
Number of wavelengths in the adjustable light Y
Number of underlying pigments Y N

23. Now add a 3rd adjustable wavelength to the light on the right

24. Person whose eye has two cone types (pigments)
Matching task: Can we adjust the amount of light in each light so that the lights look identical? If “yes,” then wavelength and intensity (amount of light) cannot be distinguished. If “no,” then wavelength and intensity can be distinguished. l1
l2 + l3+l4
Set-up: Light with one
wavelength compared to
light with three wavelengths.
Eye
Person whose eye has two cone types (pigments)
Blow-up diagram of small portion of
retina – suppose there are three cone
types, meaning 3 pigments

25. Summary Table 1 2 3 4 5 6 Y Number of underlying pigments N Y N Y
As the wavelength intensity is adjusted, is there any place where the two lights exactly match?
Number of wavelengths in the adjustable light Y
Number of underlying pigments N Y N Y

26. Now add a 4th underlying pigment
Now add a 4th underlying pigment. Will you ever get a match between the lights if there are 3 intensity adjustable wavelengths added together in the light on the right?

27. What would the table look like if we kept filling it in?