1. CHARACTERISTICS OF CELL LINE:-

2. NORMAL CELL TRANSFORMED CEL CONTINUOUS CELL
TYPES OF CELL LINE:-
NORMAL CELL
TRANSFORMED CEL
CONTINUOUS CELL

3. NORMAL CELL LINE:-
It is defined as the cell line which divides a limited number of times before loosing their ability to proliferate.
CELL LINE
CELL TYPE
ORIGIN
SPECIES
MARKER C7
Endocrine
Hypothalamus
Mouse
Neurophysin

4. CHARACTERISTICS OF NORMAL CELL LINE:-
Susceptible to human viral infection
These cell have the ability to reproduce correctly, stop reproducing whenever necessary.
Remain in specific location
Become specialized for specific function
Self destruct when necessary.

5. It can be caused by exposure to a mutagen or carcinogen.
TRANSFORMED CELL:-
It is defined as the conversion of normal healthy cell into a TUMOROUS or CANCEROUS cell.
It can be caused by exposure to a mutagen or carcinogen.
For e.g.:- Ethidium Bromide, Formaldehyde, DEPC ( Di ethyl pyro carbonate), UV radiation
Proto oncogenes are normal healthy functional genes, which have the potential to get converted into oncogenes upon exposure to a carcinogen.
E.g.-
CELL LINE
CELL TYPE
ORIGIN
SPECIES
MARKER
GH3
Endocrine
Pituitary Tumour
Rat
Growth hormone

6. CHARACTERISTICS OF TRANSFORMED CELLS:-
IMMORTALIZATION: The tumour/cancer has indefinite life span either due to mutation or deletion of senesce genes.
2. It may also occur due to mutation in proto oncogenes or over expression of oncogenes.
ABERRANT GROWTH CONTROL: The cells will lose the growth control i.e. they will grow beyond saturation density and will form heaps of cells on a confluent monolayer.
2. The cells may be immortal but still have there growth control intact.
3. E.g.- 3T3 mouse cell lines

7. 1. It is the property to invade normal healthy cell.
METASTASIS:-
1. It is the property to invade normal healthy cell.
2. E.g.- Leukaemia, blood cancer
3. Due to this property the tumour is able to proliferate at much higher rate

8. CELL SURFACE MODIFICATION:-
HIGH PLATING EFFICIENCY:-
They grow at a very high rate hence make the plate confluent in low time.
CELL SURFACE MODIFICATION:-
1. L-CAM’s are very less expressed that is cell to matrix/ substrate interaction is inhibited.
2. N- CAM’s are over expressed i.e. cell to cell interaction increase and the heaps of cells are formed.

9. SHORTER POPULATION DOUBLING TIME:-
Due to very short doubling time the tumour will replicate at a rapid rate.
GROWTH FACTOR:-
The tumour is very less dependent upon the serum and the growth factor of the host, because tumour secretes its own growth factor
TiGF
TAGF

10. CONTINUOUS CELL LINE:-
When a normal cell line undergoes transformation and acquires the ability to divide indefinitely then it is known as continuous cell line.
CELL LINE
CELL TYPE
ORIGIN
SPECIES
MARKER
MDCK
Epithelium
kidney
Dog
Domes, transport

11. CHARACTERISTICS OF CONTINUOUS CELL LINE:-
Fast growth
Grow more in suspension condition
Ability to grow up to higher cell density
Different in phenotype from donor tissue
Homogeneous cell population
Smaller, more rounded, less adherent with a higher nucleus/ cytoplasm ratio.