1. Volume 44, Issue 6, Pages 742-747 (December 2003)
Down-Regulation of Estrogen Receptor β and Transcriptional Coregulator SNURF/RNF4 in Testicular Germ Cell Cancer 
Sirpa J Hirvonen-Santti, Antti Rannikko, Henrikki Santti, Saija Savolainen, Marcus Nyberg, Olli A Jänne, Jorma J Palvimo 
European Urology 
Volume 44, Issue 6, Pages (December 2003)
DOI: /S (03)

2. Fig. 1
Individual normal testis-testicular germ cell cancer pairs shown for (A) normalized levels of ERβ mRNA in seminomas (n=8, p=0.017), (B) SNURF/RNF4 mRNA in all testicular tumors (n=12, p=0.034), (C) AR mRNA in all testicular tumors (n=12, p=0.034), and (D) cyclin D2 mRNA in all testicular tumors (n=12, NS). The mRNA level in each normal sample is set as 1, and that in tumor is shown in relation to the corresponding normal sample. Each value represents the mean of three separate analyses.
European Urology  , DOI: ( /S (03) )

3. Fig. 2
Visualization of ERβ and SNURF/RNF4 proteins in normal human testis and seminoma. Tissue specimen were stained using anti-ERβ antibody (1:1000) or anti-SNURF antibody (1:1000). (A) The presence of ERβ in spermatogonia, spermatocytes, and round spermatids in normal human testis. Positive immunoreactivity is indicated by a brown color, and cell nuclei that are negative to ERβ staining are evident by blue counterstaining. (B) Anti-SNURF immunoreactivity in Sertoli and Leydig cells, as well as in round and elongating spermatids in normal human testis. (C) Control staining of normal human testis with preadsorbed anti-SNURF antibody. (D) Anti-ERβ staining of pT1 seminoma from patient 1. (E) Anti-SNURF antibody staining of pT1 seminoma from patient 1.
European Urology  , DOI: ( /S (03) )