1. Inhibition Zone Diameter (mm) a Gram-Positive Bacteria
table 7. The inhibition zone diameter methodological analysis of the effect of toilet bowl cleaners/bleaches/sanitizers (Class B) on the growth of gram-positive bacteria
Microorganism
Inhibition Zone Diameter (mm) a
control ddH20
control Pure Methanol
undiluted Disinfectant
Antiseptic DF
1/2
1/4
1/8
1/16
1/32
Novobiocin
(30 g)
class B – Toilet Bowl Cleaners/Bleaches/Sanitizers §
Mr. Muscle Toilet Cleaner Duck
Active ingredients –Aqua, Tetrasodium EDTA, Butoxydiglycol, C9-11 pareth-6, Benzalkonium chloride, Sodium hydroxide, Parfum, Trisodium NTA, 3-(Trimethoxysiyl)-propyldimethyloctadecyl ammonium chloride, Benzyl salicylate, Alcohol, (3-Chloropropyl) trimethoxysilane, Methyl alcohol, Linalool, Lactic acid (2.02 g/100 g), Limonene, and Dimethyl stearamine.
Gram-Positive Bacteria
Bacillus subtilis
NI * NI 
16.00
 0.45
Enterococcus Group D
(E. faecalis)
8.00
 0.33
8.33
9.00
 1.73
17.00
 0.58
Staphylococcus aureus
25.33
 0.20
23.33
 0.38
 1.15
14.33
 1.07
12.00
 0.67
10.00
36.00
 1.25
Streptococcus Group A
(GAS; S. pyogenes)
9.33
 0.39
 1.92
30.00
 1.57
Streptococcus Group B
(GBS; S. agalactiae)
 0.19
7.50
 1.45
0.00
 0.00
a Mean value ± SEM, n = 3 (the zone of inhibition [mm] including disk of 5 mm in diameter).
* NI = No Inhibition; TI = Total Inhibition (the zone of inhibition [mm] including disk of 5 mm in diameter is > 50 mm); DF = Dilution Factor.
§ Commercial brands are disclosed in accordance with ethical and propriety issues.
Niveen M. Masri et al. The Immunomodulatory, Antimicrobial and Bactericidal Efficacy of Commonly Used Commercial Household Disinfectants, Sterilizers and Antiseptics in Vitro: Putative Anti-Inflammatory Infection Control Mechanisms and Comparative Biochemical Analysis of the Microbial Growth of Gram-Positive Bacteria. American Journal of Medical and Biological Research, 2013, Vol. 1, No. 4, doi: /ajmbr-1-4-4
© The Author(s) Published by Science and Education Publishing.