1. Results of Recent EQA Panels for Blood Borne Viruses
Vivienne James
UK NEQAS for Microbiology
14th June 2006
SoGAT XIX

2. EQA for Blood Borne Viruses
HIV RNA quantification
Pilot distributions started in 1998
The first distribution was sent out in September 2000
156 participants
HCV RNA detection
Pilot distributions started in 2000
The first distribution was sent out in July 2001
165 participants
HBV DNA quantification
Pilot distributions started in October 2002
The first distribution was sent out in September 2003
98 participants
CMV DNA quantification
Pilot distributions started in 2004
First distribution scheduled for 2007
55 participants
14th June 2006
SoGAT XIX

3. Report format
14th June 2006
SoGAT XIX

4. HIV1 RNA Quantification
Distribution
Specimen
Sub
type
Median concentration
cp/mL (log)
Median log10 difference
Total <0.5 log
1763 May 2004
7018 B
4696 (3.6)
0.59
116/119 (97.5)
7019
1228 (3.0)
1802 Aug 2004
7168
8187 (3.9)
1.00
116/127 (91.3)
7169
777 (2.8)
1843 Jan 2005
7328
8330 (3.9)
1.30
118/130 (90.8)
7329
388 (2.6)
1897 May 2005
7485
1780 (3.3)
0.53
117/124 (94.4)
7486
546 (2.7)
1932 Aug 2005
7602
3170 (3.5)
1.03
128/135 (94.8)
7603
332 (2.5)
1977 Jan 2006
7767
10318 (4.0)
1.06
132/138 (95.7)
7768
965 (3.0)
All specimens sent within range of 2.5 to 4 log copies/mL
Performance good however is it too easy?
14th June 2006
SoGAT XIX

5. 14th June 2006
SoGAT XIX

6. Performance criteria
14th June 2006
SoGAT XIX

7. 14th June 2006
SoGAT XIX

8. HBV DNA Quantification
Distribution
Specimen
Genotype
Median conc.
kcp/mL or kIU/mL
Median Log difference
Total <0.5 log
1807
Sep 2004
7181 A
9622
1.47
46/55 (83.6)
7182
340
7183 25
1.07
54/60 (90)
7184
2.3
1857
Mar 2005
7379
15100
0.53
52/55 (94.5)
7380
4490
7381
146.5
0.99
61/64 (95.3)
7382 15
1944
Sept 2005
7643
D (ayw) 10
0.52
66/69 (95.7)
7644
3.06
7645
4400
1.02
58/65 (89.2)
7646
519
1999
March 2006
7844 D
1.32
0.51
69/72 (95.8)
7845
0.79
7846
4.63
1.45
49/57 (86.0)
7847
3.24
14th June 2006
SoGAT XIX

9. 14th June 2006
SoGAT XIX

10. Performance criteria
14th June 2006
SoGAT XIX

11. Median concn IU/mL (log)
HCV RNA Detection
Distribution
Specimen numbers
Result
Genotype
Median concn IU/mL (log)
Correct reports
1784 June 2004
7110
HCV RNA positive 1a
971.5 (2.99)
136/143 (95.1%)
7111
HCV RNA negative
134/143 (93.7%)
1828 Nov. 2004
7274 2a
20250 (4.31)
148/149 (99.3%)
7275
1570 (3.20)
147/149 (98.6%)
1910 June 2005
7531 1b
(5.14)
154/154 (100%)
7532
12528 (4.10)
151/154 (98.0%)
1966 Nov. 2005
7731
318 (2.50)
135/157 (86.3%)
7732
2530 (3.40)
154/157 (98.0%)
1910 specimen pair 86% reported to within 0.5log of median difference
14th June 2006
SoGAT XIX

12. 14th June 2006
SoGAT XIX