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Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small.

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Presentation on theme: "Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small."— Presentation transcript:

1 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 1. The expression levels of miR-135a and IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 mRNA in the NSCLC tissues and the adjacent normal tissues, detected by qRT-PCR. **, P < 0.05 compared with the adjacent normal tissues; miR-135a, microRNA-135a; IGF-1, insulin-like growth factor-1; PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; qRT-PCR, quantitative real-time polymerase chain reaction. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

2 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 2. The positive expression of IGF-1, PI3K, Akt and CD34 proteins in the NSCLC tissues and the negative expression of these proteins in the adjacent normal tissues, detected by IHC in (× 400). A, positive IGF-1 protein in the NSCLC tissues; B, negative IGF-1 protein in the adjacent normal tissues; C, positive PI3K protein in the NSCLC tissues; D, negative PI3K protein in the adjacent normal tissues; E, positive Akt protein in the NSCLC tissues; F, negative Akt protein in the adjacent normal tissues; G, positive CD34 protein in the NSCLC tissues; H, negative CD34 protein in the adjacent normal tissues; IGF-1, insulin-like growth factor-1; PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; IHC, immunohistochemistry. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

3 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 3. The expression levels of miR-135a and IGF-1 mRNA in human HBE cells, A549, H460 and H1299 cells, detected by qRT-PCR. *, P < 0.05 compared with the HBE cells; #, P < 0.05 compared with the A549 cells; miR-135a, microR-NA-135a; IGF-1, insulin-like growth factor-1; qRT-PCR, quantitative real-time polymerase chain reaction. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

4 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 4. IGF-1 verified as a target of miR-135a. A, Target Scan software was used to predict miR-135a with binding sites in the 3’ untranslated region (UTR) of IGF-1 mRNA; B, detection of luciferase reporter gene activity: in the A549 cells cotransfected with miR-135a mimics and IGF-1 3’UTR Wt/Mut plasmids, miR-135a inhibited the luciferase activity of Wt plasmid but failed to affect the luciferase activity of Mut. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

5 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 5. The proliferation of A549 cells evaluated by MTT assay among blank, NC, miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. *, P < 0.05 compared with the blank group; #, P < 0.05 compared with the IGF-1 siRNA group. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

6 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 6. The apoptosis of A549 cells evaluated by flow cytometry among the blank, NC, miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. *, P < 0.05 compared with the blank group; #, P < 0.05 compared with the IGF-1 siRNA group. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

7 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 7. The migration of A549 cells evaluated by scratch test among the blank, NC, miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. *, P < 0.05 compared with the blank group; #, P < 0.05 compared with the IGF-1 siRNA group. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

8 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 8. The invasion of A549 cells evaluated by Transwell assay among the blank, NC, miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. A, cell invasion figures in each group; B, the number of cells penetrating into membrane in each group; *, P < 0.05 compared with the blank group; #, P < 0.05 compared with the IGF-1 siRNA group. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

9 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 9. The expression levels of IGF-1, PI3K and Akt mRNA and protein among the blank, NC, miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. A, the expression levels of miR-135a and IGF-1, PI3K and Akt mRNA; B, gray values of IGF-1, PI3K and Akt protein bands; C, the expression levels of IGF-1, PI3K and Akt protein; *, P < 0.05 compared with the blank group; #, P < 0.05 compared with the IGF-1 siRNA group; miR-135a, microRNA-135a; IGF-1, insulin-like growth factor-1; PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; qRT-PCR, quantitative real-time polymerase chain reaction. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

10 Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer Cell Physiol Biochem 2017;42:1431– DOI: / Fig. 10. The expression levels of VEGF, bFGF and IL-8 mRNA and protein among the blank, NC, miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. A, the expression levels of VEGF, bFGF and IL-8 mRNA; B, gray values of VEGF, bFGF and IL-8 protein bands; C, the expression levels of VEGF, bFGF and IL-8 protein; *, P < 0.05 compared with the blank group; #, P < 0.05 compared with the IGF-1 siRNA group; miR-135a, microRNA-135a; VEGF, vascular endothelial growth factor; bFGF, basic fibroblast growth factor; qRT-PCR, quantitative real-time polymerase chain reaction. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

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