1. BIOTECH Start-up for Phyto-DIAGNOSTICS & Smart-farming
Rishiraj Raghuvanshi
M.Sc. Previous
Dept of Plant Molecular Biology & Biotechnology
Indira Gandhi Krishi Vishwavidyalaya,
Raipur , (C.G.),

2. Lateral Flow Devices (LFD) For diagnosis of plant pathogenic diseases
Biodegradable Nutri-Releasing mulch film

3. CONTENT
Lateral Flow Devices (LFD) For diagnosis of plant pathogenic diseases-
Introduction
Procedure
Test Interpretation
Advantages

4. Introduction
Lateral Flow Devices (LFD) are for rapid, on-site and field diagnosis of viral, bacterial and fungal disease in plants.
User-friendly test, growers, inspectors and consultants are able to make instant, reliable decisions in minutes.
Lateral Flow Tests can operate as either competitive or sandwich ELISA.

5. LIA can be applied for… LIA based devices in plants for
Lateral flow immunochromatographic assays (LIAs) present for Diabetes/Pregnancy test
LIA simple colour change detection of presence or Absence of a target analyte.
LIA based devices in plants for
-FARMER/Rural
-Workers/Extension
-field Officers
Diagnose of the pathogen
On field
At Early stage
With Accuracy
TB detection

6. How to Use Select the sample
Cut or tear sample into small pieces and put into bottle
Shake firmly for seconds to break up the sample
Draw liquid into pipette (avoid sample debris and air bubbles)
Keeping the test device level, add 2 drops into the sample well of the device
Valid results within 10 minutes

10. Test Interpretation
One line present only at the C line indicates a negative result.
Two lines present, at both the T and C line indicates a positive result.
The absence of a line at C indicates the test has failed and must be repeated.

11. Advantages
You can make a rapid diagnosis and to ensure healthy crops without waiting for laboratory test results.
It doesn't require the need for specialized and costly equipment
It will help to maintain healthy crops, plants and stocks.

12. II. Biodegradable Nutri-Releasing mulch film

13. II. Biodegradable Nutri-Releasing mulch film
Mulching –at present known as Compostable film
NPK – micronutrient: Release with time in response to sun-light/ Irrigation etc.
Area of research

14. Introduction
Produced by the biomass ( plant starch) with addition of plant nutrient.
suitable for automated precision farming & sustainable agriculture
Do not requiree removal and disposal operation from the field
Alternative to polyethylene - non recyclable waste thus decreasing the environmental pollution

15. Comparative study of plastic & bioplastic film

16. Degradation of bioplastic film

17. Better yield reported with compostable Mulch

18. Conclusion Promoting organic farming & sustainable development
need to evolved technical devices
reducing the economic cost associated with thesee operations

19. Cont… Products of future Agricutlure Early Precise Diagnostic
Automated – precision farming

20. THANK YOU