1. University of Hohenheim
Genetic diversity of Echinococcus multilocularis – comparative results from mitochondrial and microsatellite markers
Sandra Schroer, Jenny Knapp, Bruno Gottstein, Anke Dinkel, Thomas Romig
University of Hohenheim

2. Sandra Schroer, University of Hohenheim
Background
Mitochondrial markers
18 haplotypes worldwide in 3 clusters (analysis of complete cob, nad2, cox1 (Nakao et al. 2009))
additional haplotypes found in Europe (various authors) with shorter fragments (cob, cox1, nad1, nad2, atp6)
N1+N2
E1- E5
A1- A10
Sandra Schroer, University of Hohenheim

3. Sandra Schroer, University of Hohenheim
Background
Microsatellite markers
Systematic study with EmsB in Europe (Knapp et al. 2009)
571 isolates  32 profiles
Proposition concerning epidemiology: mainland- island- hypothesis
Sandra Schroer, University of Hohenheim

4. Sandra Schroer, University of Hohenheim
Aim of the study
Here: Analysis of 358 samples with EmsB and mt markers
(fragments of atp6, cox1 and nd1 (~1600bp))
Aim:
Analysis of genetic diversity in
Europe
Comparison of diversity in core area
and periphery
Same/ similar/ different results
by direct comparison of markers?
Sandra Schroer, University of Hohenheim

5. Sandra Schroer, University of Hohenheim
Results
In total: 23 EmsB- profiles
Sandra Schroer, University of Hohenheim

6. Sandra Schroer, University of Hohenheim
Results
In total: 73 mt haplotypes
Sandra Schroer, University of Hohenheim

7. Sandra Schroer, University of Hohenheim
Results
Haplotype diversity index (Nei 1987)
Sandra Schroer, University of Hohenheim

8. mitochondrial haplotypes
Results
Ubiquitous and country- specific variants (n and %)
EmsB- profiles
mitochondrial haplotypes
ubiquitous
specific
Switzerland
2 (12,1%)
5 (6,06%)
1 (33,3%)
15 (66,6%)
Austria
2 (35,7%) /
1 (47,3%)
21 (25,2%)
Germany
2 (32,4%)
3 (21,6%)
1 (70,8%)
10 (12,0%)
Poland
2 (36,7%)
4 (9,2%)
1 (24,5%)
15 (17,3%)
Slovakia
2 (64,8%)
1 (54,0%)
3 (18,0%)
Sandra Schroer, University of Hohenheim

9. Sandra Schroer, University of Hohenheim
Conclusion
High microdiversity both of mitochondrial markers (73 / 358) and EmsB profiles (23 / 358)
In both systems: few widespread and numerous rare variants
Rare variants are possibly locally restricted and may be useful for geographic studies
No apparent correlation between certain mitochondrial haplotypes and microsatellite profiles
Geographic distribution of diversities disagree (Germany!)
EmsB: highest diversity in Switzerland and Germany
mt markers: diversity low in Germany, high in Poland and Austria
Sandra Schroer, University of Hohenheim

10. Thanks for your attention!
Sandra Schroer, University of Hohenheim