1. Actn3 KO mice display a shift toward more oxidative pathways of metabolism Enzyme analyses show increased expression of enzymes involved in the glycolysis pathway (PFK, HK, and GAPDH) in the Actn3 KO mice.
Actn3 KO mice display a shift toward more oxidative pathways of metabolism Enzyme analyses show increased expression of enzymes involved in the glycolysis pathway (PFK, HK, and GAPDH) in the Actn3 KO mice. The anaerobic conversion of pyruvate to lactate by lactate dehydrogenase (LDH) is reduced, whereas the activity of enzymes involved in mitochondrial oxidative metabolism [citrate synthase (CS), succinate dehydrogenase (SDH), cytochrome c oxidase (CCO)], and fatty acid oxidation [3-hydroxyacyl-CoA dehydrogenase (BHAD), and medium chain acyl-CoA dehydrogenase (MCAD)] are increased in Actn3 KO mice. Glycogen content is increased in Actn3 KO mice, and glycogen phosphorylase activity is decreased. Hexokinase activity (HK) is also increased in Actn3Kos, whereas glucose-6-phosphate (G6P) and Phsosphofructokinase (PFK) are not. NADH tetrazoleum reductase (NADH-TR) levels are increased in Actn3KOs by staining.
Yemima Berman, and Kathryn N. North Physiology 2010;25:
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