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Sheila Chandrahasa, DMD, Peter E. Murray, PhD, Kenneth N. Namerow, DDS 

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Presentation on theme: "Sheila Chandrahasa, DMD, Peter E. Murray, PhD, Kenneth N. Namerow, DDS "— Presentation transcript:

1 Proliferation of Mature Ex Vivo Human Dental Pulp Using Tissue Engineering Scaffolds 
Sheila Chandrahasa, DMD, Peter E. Murray, PhD, Kenneth N. Namerow, DDS  Journal of Endodontics  Volume 37, Issue 9, Pages (September 2011) DOI: /j.joen Copyright © Terms and Conditions

2 Figure 1 Histology of metabolically active cells from mature human dental pulp that proliferated into (A) polymer, (B) collagen, and (C) calcium phosphate tissue engineering scaffolds. The cells were observed proliferating into the tissue engineering scaffolds after 30 days of culture in contact with the dental pulp from mature tooth slices. The metabolically active cells were stained with neutral-red dye. The larger areas of dark staining are clusters of proliferating cells. Journal of Endodontics  , DOI: ( /j.joen ) Copyright © Terms and Conditions

3 Figure 2 Histology of stem cells from human exfoliated deciduous teeth that proliferated into (A) polymer, (B) collagen, and (C) calcium phosphate tissue engineering scaffolds after 30 days of culture. The metabolically active cells were stained with neutral-red dye. The larger areas of dark staining are clusters of proliferating cells. Journal of Endodontics  , DOI: ( /j.joen ) Copyright © Terms and Conditions

4 Figure 3 Proliferation of mature human dental pulp into tissue engineering scaffolds. Calcium phosphate scaffolds: average rate of cell proliferation: mature dental pulp cells per day (R2 = .549), collagen scaffolds: average rate of cell proliferation: cells per day (R2 = .910), and polymer scaffolds: average rate of cell proliferation: cells per day (R2 = .868). R2 is the coefficient of linear regression correlation between the data and the average rate of cell proliferation. Journal of Endodontics  , DOI: ( /j.joen ) Copyright © Terms and Conditions


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