1. STEM CELL ITS THERAPEUTICS AND REGENRATIONBY
DR. VIDHATRI TIWARI
1ST YEAR P.G STUDENT
PEOPLES COLLEGE OF DENTAL SCIENCES AND RESEARCH CENTRE
Presented at Paradoxes Sunday School Class, Sierra Madre Congregational Church, June 27, 2004.

2. CONTENT INTRODUCTION OF STEM CELL THERAPEUTICS IN DENTISTRY
POTENTIAL RESEARCH ON DENTISTRY
POTENTIAL RESEARCH AREAS
CONCLUSION
REFERENCES

3. INTRODUCTION: Stem cells are the "master" cells of the body.
Stem cells are defined by their ability to make many different types of cells, and to divide many more times than regular cells.

4. PROPERTIES: Self renewal
THE ABILITY TO THROUGH NUMEROUS CYCLE OF CELL DIVISION
Potecy
CAPACITY TO DIFFRENTIATE TO SPECIAL CELL

6. Give rise to fully functional organism as well to any cell type
TOTIPOTENT STEM CELL
Give rise to fully functional organism as well to any cell type
TYPES
BLOOD STEM CELL OTHER COMMITED
STEM CELL
PLATELETS ERYTHROCYTE
WBC
PLURIPOTENT STEM CELL
Give rise to most but not all tissues of an organisms
MULTIPOTENT STEM CELL:
Commited , give rise to specific cell function

7. SOURCES
ADULT STEM CELL
EMBRYONIC STEM CELL

8. Reprinted with permission of Do No Harm
Reprinted with permission of Do No Harm. Click on image for link to website.

9. Adult stem cell ADULT STEM CELL
BONE MARROW PERIPHERAL BLOOD SKELETAL MUSCLE BRAIN
MARROW BONE MARROW SKELETAL MUSCLE BRAIN
BONE BLOOD CELL SMOOTH MUSCLE NERVE
CARTLAGE NERVES CARTILAGE BLOOD VESSEL
TENDON FAT ALL TISSUES
MUSCLE HEART
FAT
LIVER CORNEA
BRAIN/LIVER HAIR FOLLICLE GIT RETINA
BLOODCELL PANCREAS
HEART
ALL TISSUES SKIN BRAIN SMALL INTESTINE LIVER
SMOOTH MUSCLE LIVER HEART
STEM CELL STOMACH LUNG
FROM FAT AMNOTIC FLUID

10. ARTICLE:
J.J MAO , WV GINNOBILE. JA HELMS, SJ HOLLISTER , P.H. KREBACH, MT LONGAKER AND S. SHI : CRANIOFACIAL TISSUE ENGEENERING BY STEM CELL: JOURNAL OF DENTAL RESEARCH: 2006:85:966.

11. MAO et al conducted the craniofacial tissue engineering promising regeneration of dental, oral, craniofacial from mesenchymal cell.

12. THERAPEUTICS IN DENTISTRY
TOOTH REGENRATION
BONE REPAIR
PERIODONTAL REGENRATION
OTHERS

13. DENTAL PULP AND STEM CELL (DPSC)
Regenerative capacity of
dental pulp are
analogous
to bone cell.
DPSC shows higher
proliferating rate
than bone
marrow.

14. STEM CELL FROM HUMAN DECIDIOUS EXFOLIATED TEETH: (SHED)
Studies have shown that
SHED is more distinctive
Than DPSC with respect to
odontogenic differentiation.

16. PERIODONTIUM REGENERATION:
Periodontal stem cell have
the potential for the forming
PDL ,cementum like
structure.
Gene transfer can improve
periodontal defects.
Surgically created wound
in alveolar bone can
regenerate PDL or
cementum.

17. HARVEST BONE MARROW OR DENTAL PULP:

18. TOOTH BUD REGENERATION:

19. Enamel organ epithelia and dental papilla mesenchyme tissues contain stem cells during postnatal stages of life

20. Late cap stage and bell stage tooth organs contain stem cells.

21. Odontogenic adult stem cell respond to mechanical as well as chemical signals.
Presumbly adult bone marrow and dental pulp tissue contain odontogenic stem cell.

22. Epithelial-mesenchymal interactions are pre-requisite for tooth regeneration.

23. POTENTIAL WORK REVIEWED IN FIELD OF DENTISTRY

24. Ohanama et al conducted a study on tissue engineering of Murine teeth and gave the creation of artificial embryonic tooth from cultured cell that can replace missing teeth following transplantation on the adult mouth.

25. Dauilib et al reported on bioengeenerd tooth from culture of tooth bud cell.Tooth bud cell from 3-7 day post natal rat for tooth tissue engeernering application can maintain in vitro for 6 days.

26. Honda et al conducted the study on enamel dentin and dentin cementum complex structure formation in tooth – tissue regeneration of porcine tooth cell in the early stage of crown formation seeded on to scaffold.

27. POTENTIAL RESEARCH AREAS

28. :
Design of conditions for maintaining the cells , pluripotency or their multipotency

29. Use of stem cells to understand the genetic mechanisms that regulate development of orofacial structure.

30. Understanding the mechanisms that regulate self-renewal of stem cells in the oral structures.

31. Use of stem cell lines to identify the conditions that are required for a human embryonic stem cell that make up the different tissues of the orofacial complex.

32. Identification of the optimal type of stem cell for orofacial diseases and disorders.

33. Use of animal model systems of oral, dental and craniofacial diseases and disorders for screening and comparing the functional capabilities of implanted human stem cells and their progeny

34. Conclusion:
The advancement of the science has transformed our lives in a way that have been unpredictable just a half century ago .A thorough understanding of the various biological process involved in repair will be pivotal to future success in developing these approaches .

35. We hope that that this report has offered an approach that balances the promise of human stem cell with the public concern about such research in the manner that will lead to consensus on how best to proceed .

36. REFERENCES:
Yen AH, Sharpe PT (January 2008). "Stem cells and tooth tissue engineering". Cell Tissue Res. 331 (1): 359–72.
NIDCR Tissue Engineering and Dental and Craniofacial Regenerative Medicine Program; Nadya Lumelsky; February 25, 2008.
NIDCR Stemcells in development/Repair of Orofacial Structures;Stan Gronthos; Sonoyama W, Liu Y, Fang D, Yamaza T, Seo B-M, et al. (2006) Mesenchymal Stem Cell-Mediated Functional Tooth Regeneration in Swine. PLoS ONE 1(1): e79.
Chai Y, Slavkin HC (2003) Prospects for tooth regeneration in the 21st century: a perspective. Microsc Res Tech 60: 469–479.
Lin NH et al;Putative stem cells in regenerative human periodontium;J Periodont Res 2008;43:
Ohta S et al ;The behaviour of stem cells and progenitor cells in the periodontal ligament during wound healing as observed using immunohistochemical methods;J Periodont Res 2008;43:
Batouli S et al; Comparison of Stem-cell-mediated Osteogenesis ans Dentinogenesis;J Dent Res 2003;82(12):
Nagatomo K et al; Stem cell properties of human periodontal ligament cells;J Periodont Res 2006;41:
Izumi K,Tobita T and Feinberg SE;Isolation of Human Oral Keretinocyte Progenitor/Stem Cells;J Dent Res 2007;86(4):
Westbrook SD,Paunovich ED and Freytes CO;Adult hemopoietic stem cell transplantation;J Am Dent Asso.2003;134(9):