1. Morphological Characters
ISOLATION OF MULTIPLE DRUG RESISTANT Citobacter sp. AR2 FROM GANGES WATER
Atanu Roy1*, Anuradha Banerjee1, Diganta Sarkar1, Prateeti Basu2, Rima Roy2 and Madhumita Roy1
1Department of Biotechnology, Techno India University, Salt Lake Campus Kolkata
2Department of Microbiology, Techno India University, Salt Lake Campus, Kolkata
Introduction
In 2007 River Ganga has been declared as the fifth most polluted river in the world1,2.
Dumping of industrial and municipal sewage has made the river water a breeding ground for multiple antibiotic resistant coliforms 3,4.
This present study was conducted to study the presence of multi drug resistant coliforms in Ganges water collected from two heavily populated ghats of Kolkata and Howrah: Dakhinweswar and Belur.
Results and Discussion
Growth curve of Citrobacter sp.AR2
 Morphological Characters
Citrobacter sp. AR2
Colour of colony
Creamish
Cell shape
Rod
Gram staining
Negative
Motility
Motile
PCR product of 16s rDNA.
Accession number is KU
Sl. No.
Antibiotics
Citrobacter sp. AR2
A.    Hexa G Plus 6 1
Ampicillin R 2
Chloramphenicol 3
Penicillin G 4
Streptomycin
0.85 5
Sulphatriad 6
Tetracycline
0.9
B.     Hexa G Minus 2 7
Ceftazidime
0.98 8
Ciprofloxacin 9
Amikacin
1.6 10
Nitrofurantoin 11
Netillin
1.8 12
Nalidixic acid
C.    Hexa G Minus 3 13
Co-Trimoxazole 14
Amoxyclav 15
Gentamicin
1.4 16 17
Ofloxacin 18
Cefuroxime
D.    Hexa G Minus 5 19
Cefotaxime
1.1 20
Levofloxacin 21
Aztreonam
1.2 22
Imipenem 23
1.5 24
E.    Hexa G Minus 1 25 26 27 28 29 30
Tobramycin
Change in pH due to growth of the organism
Future Work
Plasmid mediated drug resistance
Bioremediation properties
Materials and Methods
Samples were collected
Plated with different antibiotics by pour plate method
Tested against commercially available antibiotic discs
Biochemical tests performed
16s rDNA analysis was performed
Growth curve, MIC for major antibiotics performed
Acknowledgements
The authors would like to express their deepest gratitude to Techno India University for providing all the facilities required for this research work. They would also like to give a vote of thanks to all the faculties and scholars of Department of Biotechnology and Microbiology for their support and help all through the work.
Reference
1.  Ghosh, A., 17 October "Ganga is now a deadly source of cancer, study says",  _ 1_gall-bladder-cancer-cancer-patients-prostate.
2. CENTRAL POLLUTION CONTROL BOARD (Ministry of Environment and Forests), Govt. of India July Pollution assessment: River Ganga.
3. Levy, SB The challenge of antibiotic resistance. Sci Am. 278,
4. O’Grady F, Lambert HP, Finch RG, Greenwood D Antibiotics and chemotherapy. 7th ed. New York: Churchill Livingstone.
Table demonstrate the multidrug resistance ability of Citrobacter sp. against different antibiotics