1. From: Treatment of Inherited Eye Defects by Systemic Hematopoietic Stem Cell Transplantation
Invest. Ophthalmol. Vis. Sci ;56(12): doi: /iovs
Figure Legend:
Hematopoietic stem and progenitor cells differentiate into phagocytic cells within the cornea. (a) Representative confocal pictures of corneal sections from wild-type and Ctns−/− mice. Nuclei were stained with DAPI are seen in blue and F4/80+ cells are seen in green. (b) Quantification of F4/80-positive cells within corneas of wild-type (n = 4) and Ctns−/− (n = 4) mice. (c) Representative confocal pictures of corneal sections from Ctns−/− mice transplanted with either DsRed Ctns−/− HSPCs or DsRed wild-type HSPCs. Nuclei were stained with DAPI are seen in blue, HSPCs-derived cells are seen in red, and F4/80-positive cells are seen in green. (d) Quantification of DsRed-positive HSPC-derived cells (HSPCs), host F4/80-positive cells (F4/80), and F4/80-positive and DsRed-positive HSPC-derived cells (HSPCs F4/80) within the corneas of Ctns−/− mice transplanted with Ctns−/− DsRed HSPCs (n = 5) or wild-type DsRed HSPCs (n = 7) mice. (e) All the DsRed-expressing bone marrow–derived colocalize with the antigen presenting cells (APC) marker MHC-II class II (seen in green, upper). Few of them expressed CD11c (seen in green, lower) indicating that some of them differentiated into dendritic cells. Error bars: SEM (*P < 0.05). Scale bars: (a, c) 50 μm and (e) 30 μm.
Date of download: 11/14/2017
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