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Supplementary Figure S1
B 1800 1600 1400 1200 1000 800 600 400 200
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Supplementary Figure S2
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Supplementary Figure S3
B cover slips tissue (in DBE) spacer, made from buddies (poster) buddies
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Supplementary Figure S4
computational model fluorescence signal overlay yz xy xz
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Supplementary Figure S5
B
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Supplementary Figure S6
B C
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XY YZ XZ XY YZ XZ YZ XY XZ signal (lectin) fluorescence computational
Supplementary Figure S6 (continued) D XY YZ signal (lectin) fluorescence XZ XY YZ computational model XZ YZ XY overlay XZ
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Supplementary Figure S7
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Dependence on clearing
Supplementary Figure S8 Imaging Technique Speed Resolution Photo Bleaching Imaging Depth Dependence on clearing 2-photon +/- ++ +/- ++ + confocal + ++ + + ++ lightsheet +++ +/- +/- +++ +++
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