1. Introduction to the Microscope
Care
Parts
Focusing
Scientific Drawings
Students will have their microscopes out, for reference as we go through each of the steps. As an introduction, students will be asked what kinds of things they can do with this tool.

2. Microscope Care Always carry with 2 hands in front of your body
Only use lens paper for cleaning
Do not force knobs
Turn off microscope before unplugging
Always store covered
Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.

3. Microscope Parts Eyepiece Body Tube Revolving Nosepiece Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
Diaphragm
Fine Focus
Light
Base

4. Microscope Parts Eyepiece- contains a 10X lens
Nose piece- A rotating head that has the objective lenses attached to it.
Objectives- a casing for a lens
High (40x) and Low-Power (4x and 10X).
Multiple the objective by the eyepiece to get total magnification.
Ex. 40x * 10x = 400 total magnification
Stage and stage clips- The specimen slides rests on the stage and the stage clips hold the slide in place

5. Microscope Parts Diaphragm- controls the amount of light
Located under the stage
Fine adjustment knob- The smaller of two sets of knobs located on either side of the arm.
This adjustment is used to make small adjustments in focusing.
Course adjustment knob- The larger of two sets of knobs located on either side of the arm, just above the base
This adjustment is used to make large adjustments in focusing by moving the lenses up and down. Never use this adjustment when using the 40X objective

6. Using the Microscope Place the Slide on the Microscope
Use Stage Clips to secure slide
Click Nosepiece to the lowest (shortest) setting (4x)
Look into the Eyepiece
Use the Coarse Adjustment knob to find your specimen
Turn coarse adjustment knob to raise the stage all the way up to the objective
Slowly lower it to locate specimen
Give students a slide from the “common things” set, each student will practice focusing and changing objectives.

7. Using the Microscope Want to go in closer?
Follow steps to focus using low power
Click the nosepiece to the middle objective (10x)
Use the Coarse adjustment knob to find your specimen
Use the Fine Focus Knob to bring the image into focus
Have students exchange slides so they can look at different things, walk them through using the high power objective to focus slides. Emphasize not using the coarse objective during this process, as it will crack the slides.

8. Using the Microscope Want to get even closer?
Follow the steps to focus using 4x and then 10x
Click the nosepiece to the longest objective (40x)
Do NOT use the coarse adjustment knob
Focus by using the fine adjustment knob

9. Using the Microscope
But I want to look at my specimen up close first, why do I still have to start at 4x?
You can’t zoom in on your specimen until you know exactly where it is on the slide.
Think about when you “google a map…”

10. Using the Microscope You Google the location of Stonebriar Mall:
2601 Preston Rd
Frisco, Texas 75031
Instead of starting at 4x you start at 40x

11. Using the Microscope The equivalent to 40x on your google map is this:
Does that help you locate the mall?
It doesn’t help you when using a microscope, either

12. Using the Microscope So, start at 4x (google equivalent of 4x):
You now have the big picture to look at

13. Using the Microscope Then, move on to 10x (google equivalent to 10x):
Now you can zoom in to look at those details

14. Using the Microscope
Once you know where you are, this is the google equivalent to 40x:

15. Scientific Drawings Draw a circle to represent the field of view
Make your drawing to scale
The name of the specimen should be centered and underlined at the top of the drawing
The magnification should be centered below the drawing
Remember, multiply the power of the eyepiece lens by the power of the objective lens

16. Scientific Drawings
Lines for labels need to extend from the drawing to the right
Should be parallel and end at the same point on the page
Drawn with a ruler
Labels are printed beside the line, not above it

17. Scientific Drawings Incorrect Scientific Drawing:
40x
Anacharis