1. The Role of NVSL as an OIE Reference LaboratorY IAV-Swine & IAV-Avian
Sabrina Swenson, dvm ms phd
Diagnostic virology laboratory
U.S. Department of Agriculture
Animal and Plant Health Inspection Service
Veterinary Services
National veterinary services laboratories Ph
PAHO 10/2/17

2. OIE Provides Guidance for Reference Labs
Terms of reference
laboratories/introduction/
Develop, validate, disseminate test procedures
Develop and distribute reference materials
Provide diagnostic testing
Carry out and/or coordinate scientific studies
Provide scientific and technical training
Collect, process, analyze, publish, and disseminate epi data
Provide disease experts available for consultation

3. Dissemination of Information
Websites
information/avian-influenza-disease
information/swine-disease-information/ct_siv_surveillance
Publications
Quality assurance documents – SOPs
(avian)
(bovine, porcine, aquaculture)

4. Develop and Distribute Reference Materials
Refer to NVSL website
Reagents and Proficiency Tests Diagnostic reagents are produced by the National Veterinary Services Laboratories as needed when a commercial source of reagent is not available or when commercial sources are not fulfilling diagnostic needs. This section contains a list of available reagents, including proficiency tests, and instructions for ordering

5. Provide Diagnostic Testing
Refer to website
Requirements for international testing
Letter from the Ministry of Agriculture or equivalent authority
Need permission to test due to OIE reporting obligations
Permit to import samples (NVSL provides)
Diagnostic Testing Authorized individuals may submit specimens to the NVSL facilities for diagnostic testing. This section describes the types of tests performed at the NVSL and provides instructions for submitting samples. Includes statement regarding ownership of specimens.
NVSL Submission Forms This section contains downloadable laboratory-related forms

6. Provide Scientific and Technical Training
Refer to website
Diagnostic Training The NVSL provides a variety of courses on diagnostic testing, specimen collection, and disease recognition. This section describes courses and instructions to register for a course.
Customized training
At NVSL
Not listed in training catalog
Alternatives
Twinning through OIE
Unique on-site training that is not managed through OIE

7. Disease Experts for Consultation
Use general s depending on the species
Participate as needed in meetings, conference calls, webinars, etc.
OFFLU (
OIE (
WHO (

8. IAV-S Surveillance Voluntary participation
Endemic disease in the U.S.
Voluntary participation
NVSL is primary lab for zoonotic investigations involving swine
Results communicated to Agriculture and Public Health Authorities
Sharing of sequence and viruses between USDA and CDC

9. IAV-S Surveillance
Initial testing is through National Animal Health Laboratory Network labs
Matrix PCR and subtyping PCR
Virus isolation
Sequence H, N and deposit sequence in public domain (GenBank)
Report results to NVSL
Provide viruses to NVSL for incorporation into repository
NVSL conducts WGS on selected viruses

10. Identification of suspect variant influenza A virus1 with or without suspected swine contact
State Public Health Dept. provides specimen to CDC ID for confirmation
CDC
Influenza Division (CDC ID)
CDC ID
CDC lab confirms variant infection
VS CDC Liaison &
Commodity Program Lead (CPL)
CPL2
- Industry Partners
(AASV &NPB)
- FSIS (as needed)
State Animal Health
*AD State of origin
State/Local PH
Partners
Conclusion of investigation3
CDC Lab Result
Pos
Neg
notification to APHIS-VS Partners:
TO: Commodity Program Lead (CPL), NVSL Director, NVSL DVL Director , NVSL Bovine, Porcine and Aquaculture DVL Head, NAHLN Coordinator, VS Epi Chief, NPIC, CVB rep, ARS- NADC, AD State of Origin*, District swine contact (DSC), and OHCC.
CC: VS DA Chief of Staff, VS SPRS Chief of Staff, LPA Director, STAS Scientific Advisor, NAHLN Associate Coordinators, VS ADAs, Swine Commodity Director, CEAH Director, CEAH RI, RA and SDA Directors.

11. IAV-A Surveillance Wild bird surveillance
NVSL does confirmatory testing
Managed avian species surveillance
Healthy bird surveillance
Multiple lab options – NAHLN, NPIP
Reactor samples forwarded to NVSL for confirmation

12. Sick bird investigations
Testing may be done at NAHLN lab with State and Federal agreement
Duplicate samples to NAHLN lab and NVSL
NAHLN lab does matrix PCR and subtyping PCR
Lab reports results to NVSL
NVSL testing
Confirm PCR results
Sequencing – leverage NAHLN results
Virus isolation
Maintain repository of isolates

13. Questions?