1. Introduction to Micro Lab

2. Welcome to Micro Lab
The goal of the laboratory is to expose students to the wide variety of life in the microbial world.
Although the study of microbiology includes bacteria, viruses, algae and protozoa, this lab will concentrate primarily on the bacteria

3. Microbiological techniques are important in preparing the students for the much harder task of identifying the pathogenic microorganisms in a clinical and environmental specimen.
Each experiment with a brief theoretical introduction revealing the theoretical basis on which the experiment is based on, so that there will be a strong conjunction between the practical and theoretical sessions.
Also included in this manual, the safety precautions that each one in the field of microbiology should know and practice

4. Bacteria belong to the kingdom Monera
Bacteria belong to the kingdom Monera. This kingdom contains more biological diversity than all other kingdoms combined.
Most people tend to associate bacteria with disease, but less than ten percent of all bacteria cause disease.
Many bacteria cannot even live at the temperatures found in and on the human body.
In this lab, most of the bacteria with which we will be working are non-pathogenic (do not cause disease). However, some of the bacteria are opportunistic; that is, they can cause disease in an ill or injured person.
Therefore, treat all bacteria as if they are pathogenic (cause disease).

5. Laboratory Safety Rules
1. Wear a lab coat in lab. We will be working with a variety of materials that can cause permanent stains on some fabrics. Also, a lab coat can help protect from accidental contamination by microorganisms
No eating or drinking during lab. Many pathogens spread by ingested food and drink. In addition, food can carry microorganisms that might contaminate laboratory cultures

6. Laboratory Safety Rules
Keep long or fluffy hair tied up and out of the way. Hair can contaminate and be contaminated by microbial cultures.
Always wear shoes in lab
Thoroughly wash your hands with soap and water before and after lab. Thorough and frequent hand washing easily and effectively controls the spread of many pathogens.

7. Laboratory Safety Rules
Clean the lab bench with disinfectant before and after lab. This helps to prevent contamination of cultures, books, clothing, etc
Keep the lab bench free of unnecessary materials. Don't use the lab bench as a storage area for coats, books, etc.
Do not take cultures from the lab area

8. Laboratory Safety Rules
Dispose all contaminated materials in autoclave bags. When in doubt, ask the instructor.
Immediately report all accidents and spills to the instructor. Cover spills with disinfectant-soaked paper towels for at least 15 minutes before disposing them
Read all assigned materials before the lab session. Experiments will go smoother and have greater chances of success when you know what you will be doing ahead of time

9. Laboratory Safety Rules
Treat all microbial cultures as if they are pathogens. Better safe than sorry
When in doubt, ask the instructor.
The only stupid questions are those that are intended as such.

10. NOTES 1. Personal belongings are not to be stored in the laboratory.
2. You will be assigned to a group consisting of four students and you will work together in a semester long project

11. Lab requirements
Lab coat
Matches or lighter
Soap
Waterproof permanent marker
Small or medium size towel
Sealable plastic bag
Rubber band
General Microbiology Manual

12. Acquaint yourself with microbiology lab
Dettol
Microscope
Staining solutions
Microscopic slides
Bunsen burner

13. Acquaint yourself with microbiology lab
Forceps
Wooden tongue depressor and swabs
Sterile cups
Normal saline
Syringes

14. Acquaint yourself with microbiology lab
Incubator
Autoclave

15. Acquaint yourself with microbiology lab
Laminar flow Hood

16. Acquaint yourself with microbiology lab
Culture Plates or Petri Dishes
Flaming Nichrome loop
Plastic Loops and Needles

17. Acquaint yourself with microbiology lab
Anaerobic Jar
Candle jar

18. Acquaint yourself with microbiology lab
Anaerobic station

19. Acquaint yourself with microbiology lab
Enterotube
API 20 E

20. Acquaint yourself with microbiology lab
Liquid media in tubes
Solid media in tubes

21. Acquaint yourself with microbiology lab
Different forms of slants

22. Acquaint yourself with microbiology lab
Blood culture bottles

23. Acquaint yourself with microbiology lab
Antibiotic disks

24. Try to Identify all the tools and Equipment around you
Now
Try to Identify all the tools and Equipment around you

25. END OF LECTURE