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Forensic Science International: Genetics
Validation of a rapid DNA process with the RapidHIT® ID system using GlobalFiler® Express chemistry, a platform optimized for decentralized testing environments Susana Salceda, Arnaldo Barican, Jacklyn Buscaino, Bruce Goldman, Jim Klevenberg, Melissa Kuhn, Dennis Lehto, Frank Lin, Phong Nguyen, Charles Park, Francesca Pearson, Rick Pittaro, Sayali Salodkar, Robert Schueren, Corey Smith, Charles Troup, Dean Tsou, Mattias Vangbo, Justus Wunderle, David King Forensic Science International: Genetics Volume 28, Pages (May 2017) DOI: /j.fsigen Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 1 RapidHIT ID System. Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 2 RapidHIT ID consumables. (a) sample cartridge, (b) primary cartridge. Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 3 RapidLINK™ software. Enables data review and control of geographically dispersed RapidHIT ID systems. Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 4 Effect of lysis incubation time on average peak height and heterozygote peak height ratio. Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 5 PCR boundary study results using control swabs (n=3 per condition; average±SD). Asterisk indicates standard condition. Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 6 Effect of tobacco on control swabs (n=3 per condition) with 100, M cells (average±SD). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 7 Sensitivity study with 1000M cells applied to swabs (n=3 per condition; average±SD). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 8 Electropherograms from the sensitivity study for the male donor. Note the vertical axis scale is 0–24,000 RFU for (d)–(f) and 0–7,000 RFU from 2 swipes to 1 touch for (a)–(c). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 8 Electropherograms from the sensitivity study for the male donor. Note the vertical axis scale is 0–24,000 RFU for (d)–(f) and 0–7,000 RFU from 2 swipes to 1 touch for (a)–(c). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 9 Accuracy. Size difference (in base pairs) between an allele and its corresponding allele in the allelic ladder used to size the sample (53 samples, 2138 alleles). The color of each data point indicates the corresponding dye in the GlobalFiler® Express assay; FAM (blue), VIC (green), NED (yellow), TAZ (red) and SID (purple). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 10 Electropherograms for blank runs showing (a) and (b) primer flare peaks, (c) dye blobs, (d) anomaly peak in D2S441.For all graphs, the vertical axis is intensity in RFU and the horizontal axis is fragment length from 76 to 200bp. Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 11 Average peak height and average heterozygote peak height ratio for each of three RapidHIT ID systems and the combined result (n=5 per system; n=15 combined; average±SD). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 12 Size difference between allele and corresponding allele in allelic ladder for fifteen 1000M samples (average±SD). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 13 Intracolor balance (ICB) for fifteen 1000M samples using 3 instruments (average±SD). Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Fig. 14 Standard deviation of allele size. Data represents 10,290 total alleles from 30 allelic ladder samples on three RapidHIT ID systems. Forensic Science International: Genetics , 21-34DOI: ( /j.fsigen ) Copyright © 2017 The Author(s) Terms and Conditions
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Forensic Science International: Genetics 2017 28, 21-34DOI: (10.1016/j.fsigen.2017.01.005)
Copyright © 2017 The Author(s) Terms and Conditions
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