1. Candidate phosphopeptides Verified by mutagenesis
Table 1. Identification of novel ERα phosphorylation sites. The combined data for each phosphopeptide were sufficient to identify the phosphorylation site. Verification of sites was performed by mutagenesis and/or validated phosphoantibodies.
Phospho-peptide
Phospho-
amino acid
MED release
Candidate phosphopeptides
Identity
Verified by mutagenesis
Phospho-antibody A
Serine 5
278-GEVG (S) AGDMR-287
Serine 282 + B 4
556-GGA (S) VEETDQSHLATAGSTSSHSLQK-581
Serine 559 C 7
172-GSMAME (S) AK-180
288-AANLWP (S) PLMIK-299
450-SIILLN(S)GVYTFLSSTLK-467
521-GMEHLY(S)MK-529
Serine 294 D 10
38-PLGEVYLDS (S) KPAVYNYPEGAAYEFNAAAAANA
QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSV(S)P(S)P
LMLLHPPPQL (S) PFLQPHGQQVPYYLENEPSGYTVR-142
184-YCAVCNDY (S) GYHYGVWSCEGCK-206
Serine 47 -