1. From: Two-Photon Autofluorescence Imaging Reveals Cellular Structures Throughout the Retina of the Living Primate Eye
Invest. Ophthalmol. Vis. Sci ;57(2): doi: /iovs
Figure Legend:
(a) Nerve fiber bundles imaged using a conventional confocal reflectance modality. Dark gaps between bundles are visible. (b) Two-photon fluorescence image at λex = 730 nm, captured simultaneously. Bright streaks are visible in the two-photon image. (c) Averaged cross-section across the dark bands in (a) and the bright streaks seen in (b) for the locations marked by yellow rectangles. This shows that the dark bands in reflectance correspond to bright bands in the two photon images. Two-photon imaging conditions: average power at the cornea, 7 mW; scan size, 1.1° × 1.3°; duration of recording, 12,800 frames (582 seconds); exposure, 4823 J/cm2. Exposure was greater than MPE by a factor of 8.5. Scale bars: 50 μm.
Date of download: 12/18/2017
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