1. Diffusion & Osmosis Solvent = solute(s) + solvent
1. examine diffusion of solutes
Ex 6.3-dialysis
􀁡2. examine diffusion of the solvent(osmosis)
Eg.6.2

2. Diffusion exercise Diffusion exercise
􀁡Movement of molecules from high concentration to low concentration(down the concentration gradient)
􀁡diffusion through solid (agar plates) liquid and gas

3. Factors influencing diffusion
􀁡Rate and temperature (increase diffusion w/ increase temp)
Molecular weight (increase diffusion w/ decrease MW)
solute MW Potassium permanganate = and Janus Green =
Ex 6.1- Try different temperatures

4. Temperature and diffusion
Ex 6.1- Prepare the 2 plates of AGAR
Add a drop of each dye into the well.
Place at different temperatures
Measure the diffusion diameter of the well at different time intervals

5. Osmosis experiment Ex 6.2 Prepare bags as instructed in the book.
Place them at the respective beakers.
Check initial wt and wt periodically to see if osmosis has occured

6. Diffusion permeability of Membranes,
10 ml of solution (starch + protein + NaCl + Na2SO4)
test water after 1 hr for:
Starch w/ Iodine (blue-black)
Cl - w/ AgNO3 (milky white ppt.)
SO4
- w/ BaCl2 (white ppt.)
Protein w/ Biuret (violet)

7. Tonicity exercise plant cells (Elodea leaf cells)
Turgid􀁡hypotonic solution,􀁡water enters cell􀁡chloroplasts are at the periphery of thecell

8. Tonicity-Ex 6.4 B Plasmolyzed 􀁡hypertonic solution
􀁡water leaves the cell
􀁡chloroplasts are at the center of the cell

9. TONICITY-EX 6.4 A animal cells (erythrocytes)
􀁡normal (0.85% NaCl) isotonic, no net movement of water
􀁡crenated (10% NaCl)hypertonic,water leaves
􀁡hemolyzed (0.45% NaCl),hypotonicwater enters