1. NANOTECHNOLOGY IN FOOD AND AGRICULTURE
Enisa Omanovic-Miklicanina, Mirjana Maksimovicb
aFaculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8,
Sarajevo, Bosnia and Herzegovina
bFaculty of Electrical Engineering, University of East Sarajevo, Vuka Karadžića 30, East Sarajevo, Bosnia and Herzegovina

2. Introduction
Why?
Nanotechnology has great promise in addressing many of the pressing needs in the food and agriculture sectors.
How?
Nanotechnology improves current agri-food practices by opening new avenues of food production, manufacturing and packaging, plant cropping and animal production and fisheries, making the food and agriculture industry the most important fields of nanotechnology application.

3. Introduction

4. Nanotechnology impact on food safety
Food safety implies biological, detrimental, and chemical factors.
Food safety can be quantitatively assessed by various methods, including cell culture and fine instrumental analysis.

5. Nanotechnology impact on food safety
Methods for food safety assessment require analytical time ranging from several hours to days, usually with different pretreatment steps.
Nanospectroscopic methods (IR, Raman and SERS) present very powerful alternative to the existing methods.

6. Applications
Nanotechnology holds major implications in food and agriculture industry and it is multidisciplinary in nature

7. Applications
Agri-food nanotechnology - applied research field emerged from nanoparticles and nanotechnology aspects applied in agriculture to boost crop, livestock and food productivity.
It offers the tools to understand and transform biosystems and various solutions to agriculture and food industry in the sense of sensing and controling, diagnostics and treatment, food preparation and conservation, etc.

8. Applications
Agri-food nanotechnology enables new nanoscale materials and processes, green manufacturing, development platforms as well as promise of sustainable development in long term.

9. Applications
Merging nanodevices and Internet creates a new paradigm, Internet of Nano Things (IoNT), which holds the potential to create a completely smart IoNT-driven agriculture.
With the help of IoNT, various parameters along whole food chain can be monitored in a real-time, such as soil and crop conditions monitoring as well as perform food traceability from production, transportation to food shells.  

10. Applications

11. Nanotechnology in plant and livestock production
Nanoparticles and targeted delivery nanoformulations are broadly used for agrochemicals (nanopesticides, nanofertilizers), and as vectors for gene transport.

12. Nanotechnology in plant and livestock production
Some of the current applications of nanotechnologies in plant and livestocok production:
Nanosensors for monitoring soil conditions, pesticides, herbicides, fertilizers, insecticides, pathogens and crop growth as well,
Nanosensors for detection of food-borne contaminants or for monitoring environmental conditions at the farm,

13. Nanotechnology in plant and livestock production
Some of the current applications of nanotechnologies in in plant and livestocok production:
Nanochips for identity preservation and tracking,
Nanocapsules for delivery of pesticides, herbicides, fertilizers and vaccines that allow the dosage reducement and ensure controlled slow delivery to plants,
Bio-nanosensors to detect metabolites like lactate, glucose and ATP within animals

14. Nanotechnology in plant and livestock production
Some of the current applications of nanotechnologies in in plant and livestock production:
Aptasensors for determination of antibiotics, drugs and their residues (e.g. cocaine, oxytetracycline, tetracycline, kanamycin),
Aptasensors for determination of heavy metals (e.g. Hg2+, As3+, Cu2+).

15. Nanotechnology in food production
Nanotechnology plays a key role in achieving safe and quality food products, through detecting pathogens and contaminants by using nanosensors and indicators, encapsulating nutrients and developing new functional products.

16. Nanotechnology in food production
Applications of nanotechnology in food production:
Nanotechnology-based tracing for detection of the presence of pathogens, spoilage microorganisms, allergens, chemicals and other contaminants in food as well as nutritional information,
Nanoencapsulated flavor enhancers,
Aptasensors for determination of microbial toxins (e.g. OTA, Fumonisin B1)

17. Nanospectroscopy in food processing
Raman spectrometry has great potential in food applications (monitoring and quality control in industrial food processing, food safety etc.).
Advantages of Raman spectroscopy over other analytical methods applied in food analysis are short analysis time and ability to probe water-rich samples.
Main disadvantages are poor signal and very strong fluorescence signal which can overlap with Raman signal.

18. Nanospectroscopy in food processing
Applications:
monitoring of food processes and fermentation,
heating and oxidation of edible oils,
processing and preservation of meat,
determination of food contaminants.  

19. Nanospectroscopy in food processing
Surface enhanced Raman spectroscopy (SERS) techniques have been applied in food analysis as a rapid and sensitive tool for the detection of food contaminants and as a diagnostic tool for foodborne microorganisms.  

20. Nanotechnology in food packaging and transportation
In nano-food system, packaging plays significant role in prolonging shelf life, freshness and quality of fresh and stored food products.
Numerous nanosensors are developed for various applications in food sector, mostly for improving the shelf life, food quality, safety, fortification as well as utilizations of biosensors for contaminated or spoiled food or food packaging.

21. Nanotechnology in food packaging and transportation
Nanosensors in food packaging
Smart packaging and food tracking
Nanosensors and antimicrobial activators are being engineered to be capable of detecting food spoilage and releasing nanoantimicrobes. Such systems will extend shelf-life of food.
Mars Inc. is a company that has patented invisible edible nano wrapper which will act as an envelope for foods to prevent gas and moisture exchange.

22. Nanotechnology in food packaging and transportation
Nanosensors in food packaging
Interactive ‘smart’ food
Kraft and Nestle food companies are working on designing „smart‟ foods that are intended to interact with the consumers so they can personalize their food, by changing color, flavor, and nutrients on demand.
Kraft is developing a clear tasteless drink that contains hundreds of flavors in latent nanocapsules, by using a microwave you would be able to trigger the release of the color, flavor, concentration and texture of the individual’s choice.

23. Nanotechnology in food packaging and transportation
Nanosensors in food packaging
Aptasensors
Aptasensors are aptamer-based biosensors with excellent recognition capability towards a wide range of targets.
Nanoparticle-based aptasensors consists of aptamers (the target-recognition element) and nanomaterial (the signal transducers and/or signal enhancers).

24. Nanotechnology in food packaging and transportation
Nanosensors in food packaging
Aptasensors
Aptamers are single stranded nucleic acid or peptide molecules of size less than 25 kDa with natural or synthetic origin. They are highly specific and selective towards their target compound.
There is a wide variety of nanomaterials which can be used in aptasensors, metal nanoparticles and nanoclusters, semiconductor nanoparticles, carbon nanoparticles, magnetic nanoparticles etc.

25. Nanotechnology in food packaging and transportation
Nanosensors in food packaging
Aptasensors
Nanospectroscopic techniques Raman scattering and SERS and surface enhanced resonance Raman scattering (SERRS) have important application in construction of aptasensors applied in food packaging.

26. Nanotechnology in food packaging and transportation
Nanosensors in food packaging
Aptasensors

27. Food forensic Investigation of Food origin Authentication (fraud)
Provenance of a foodstuff
Microbial spoilage and contamination

28. Food forsenic
Food forensic involves the possibility of using powerful scientific methods for the authentication and traceability of foodstuffs.
Nanospectroscopic methods are very important in this field.

29. Food forsenic
Raman spectroscopy is ideally suited for food forensic, due to the specificity and the diversity of the analytes which can be probed; ranging from the macro-food, lipids, proteins and carbohydrates, to the minor components, dyes, pigments, preservatives.

30. Food forensic
Raman and IR spectroscopy have been applied in the identification of olive oils adulterated with hazelnut oil. 
Raman spectroscopy have been employed in the authentication of apple, pomegranate and blueberry juices. 

31. Food forensic
Raman spectroscopy has been used to confirm the botanical and geographical origins of European honey.
Methods based on IR spectroscopy are used more in the determination of microbial spoilage and contamination than Raman spectroscopy.

32. Conclusions
Nanotechnology and nanospectroscopy play an important role in agriculture and food production as well as in livestock production.
Their potential use and benefits are enormous.
Existing applications of nanotechnology in food and agriculture are more oriented to reduce the negative impact of agrochemical products in the environment and human health, rather than the utilization of nanotechnology applications to improve their properties for food and livestock production.

33. Conclusions
Vibrational spectroscopy as a nanospectroscopy method is an extremely useful tool for food industry and agriculture.
The field of food forensics is a very important and modern application of vibrational spectroscopy, especially in determination of food origin and adulteration.
Successful utilization of aptasensors in several detection formats, including food and water analysis is confirmed by plentitude of available literature related to application of aptamers.

34. Conclusions
However, to employ nanoaptasensing in food analysis, it is necessary to prioritize the ultimate aim of the application.
The potential benefits of nanotechnology for agriculture and food production need to be balanced against concerns for the soil, water, environment, and the occupational health of workers.
The success of these advancements depends of knowledge and awareness of potential toxicological effects of nanofoods, nanoagrochemicals, nanopackaging and other nanomaterials, risk of exposure, user acceptance and regulation issues.

35. THANK YOU FOR YOUR ATTENTION!!