1. Blood smear

2. Blood smear:
A drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide.
Stained with Wright’s stain.

3. Blood smear：Wright X400 Erythrocytes: 7-8um Biconcave disc
Stain uniformly with eosin
Neutrophil:
proportion %;
Most numerous
Multilobed nucleus
Pale pink granules
The method of producing a blood smear: a drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide.
A smear from peripheral blood.The cells are mainly erythrocytes.
Blood smear：Wright X400

4. Eosinophils. Blood smear：Wright X400 8-10 microns morphology
2-4%
8-10 microns
morphology
bilobed nucleus large,
uniform eosinophil granules.
Blood smear：Wright X400

5. Blood smear：Basophil Giemsa stain X400
Basophils.
a.Proportion 0.5%
b.morphology - large, irregular basophil granules obscure the nucleus
The basophil granules are very large and irregularly shaped. Granules obscure the nucleus.
Blood smear：Basophil Giemsa stain X400

6. Blood smear：Wright X400 Lymphocyte: Intensely stained nucleus
Possess a slight indentation
Small amount of cytoplasm
Pale blue
Blood smear：Wright X400

7. Blood smear：Wright X400 Monocyte nucleus ovoid to kidney shaped
blue-gray Cytoplasm
Blood smear：Wright X400

8. Blood smear：Wright X400 Platelets: Small speck-like objectives
Aggregated into small groups
From megakaryocytes in myeloid tissue.
Platelets are indicated by arrows. Some leukocytes are present.
Blood smear：Wright X400

9. Blood smear：Wright X400

10. Thymus Lymph node Spleen
Immune system
Thymus
Lymph node
Spleen

11. Capsule
Interlobular septa
Incomplete lobule
Cortex
medulla
Thymus: HEX40

12. Capsule
Interlobular septa
Incomplete lobule
Cortex
medulla
Thymus: HEX100

13. Thymocyte
Epithelial reticular cell
Thymic corpuscle
Thymus: HEX400

14. Spheroid or kidney shaped organ
Hilum
Cortex
Medulla
Lymph node

15. Capsule
Cortex
Lymphoid nodules
Paracortical zone
Cortical lymph sinuses
subcapsular
peritrabecular
Lymph node: HEX40

16. Small lymphocytes
Germinal center
young lymphocytes
reticular cells
macrophages
Lymphoid nodule

17. Postcapillary venules In the paracortical zone LC within the wall
thick endothelial cell
LC within the wall
opening for LC enter LN

18. Lymph node:HEX400 cortical lymph sinuses (subcapsular)

19. Cortical lymph sinuses: peritrabecular sinuses

20. Afferent lymphatic vessel in the capsule: with valves

21. Efferent lymphatic vessels
Blood vessels
Hilum of lymph node

22. Pacinian corpuscle
Nerve fiber bundle

23. Medullary cords
Medullary sinuses
Medulla of lymph node

24. Spleen

25. Capsule
DCT
Smooth muscle
mesothelium
Spleen

26. Trabeculae
artery
vein
Trabecular artery
Trabecular artery
Spleen

27. White pulps Lymphatic sheath lymphatic tissue central artery
Splenic nodule
Trabecular vein

28. White pulps
Lymphatic sheath
lymphatic tissue
central artery
Splenic nodule

29. Red pulps
splenic cord
Splenic sinusoid

30. Postcapillary venules in inner cortex

31. SS: subcapsular sinus
T: trabecular

32. Capsule
Subcapsular sinuses
Trabeculae

33. Afferent lymph vesselsGC

34. Older adult thymus has large amounts of adipose tissue

35. THE END!