1. Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
Altogen Biosystems offers the 293 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The 293 Transfection Reagent is an advanced formulation of a lipid-based reagent, and it has been developed to provide high transfection efficiency with the 293 cell line.
This cell line has applications for studying virucide or efficacy. When cultured in vitro, 293 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase 293 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. 293 Cell Line Description and Applications
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
293 Cell Line Description and Applications
The 293 cell line originates from tumorigenic embryonic kidney tissue from a human fetus. The cell line is also known as “Human Embryonic Kidney 293 Cells” or HEK Cells. It is a hypotriploid cell line with a modal chromosome number of 64. HEK 293 Cells grow in culture and transfect efficiently, and as a result, these cells have been a staple in biomedical research for several years. In particular, HEK 293 cells are vital for the development of therapeutic proteins and viruses related to gene therapy. With several transfection techniques, such as the calcium phosphate method, it is possible to achieve 100% transfection with the 293 cell line.
High Density 293 cells
Atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. 293 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
293 Transfection Protocol
1. Plate 10, , cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with 293 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of 293 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. 293 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
293 Transfection Kit Product Details
Two-component formulation enhances lipid-mediated transfection efficiency
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
Achieve robust siRNA uptake for dependable gene silencing
Effective transfection under conditions of up to 40% serum
Transfection kit includes Transfection Enhancer reagent
Gentle enough to be used for single cell analysis
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Data Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
Data
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the 293 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).

7. Data Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
Data
Figure 2. Protein expression of GAPDH in 293 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into 293 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. 293 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > 293 Transfection Reagent (Emb Kidney, CRL-1573)
293 Transfection Kit Benefits
Pre-optimized transfection protocol for 293 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use 293 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable 293 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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