1. BIOL 100L
Microscope & Cells

2. Microscope, Cells & Mitosis Lab
Prelab Quiz
Models: cells
Prepared slides in order
Crossed threads- just focus
Letter “e”- draw at 4, 10, 40x mag
6 slides- draw at best mag
include sperm and blood at 100x
Wet Mount slides
Pond water
Yeast
Cheek cell

3. Carrying a Microscope A B

4. Storing The Microscope
Return the lowest power objective in place
Wrap the cord around the base
Return dustcover

5. Cleaning the Microscope
Use lens paper on all glass parts of the microscope.
Clean oil immersion lens with chemicals provided by your instructor

6. Parts of the Microscope
Ocular
Body tube
Nosepiece
Objective lenses (10, 40, 100x)
Arm
Course adjustment knob
Fine adjustment knob
Stage
Stage clips
Aperture
Diaphragm
Light
Base

7. Microscope Parts Ocular Body tube Head Revolving nose piece Objective
Arm
Stage
Diaphragm
Lever to move stage clip
Course adjustment
Fine adjustment
Light source
Base

8. Using the microscope Always observe using the LOWEST POWER
Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Then use FINE ADJUSTMENT KNOB .
Focus, and then move to a higher power objective, if needed.
Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE.
Keep both eyes open to reduce eyestrain.
Determine total magnification of the object by multiplying the power of the ocular (10x) the power by the power of the objective.

9. Preparing a slide

10. Oil Immersion Lens
40x
100x
When finished: clean objective and prepared slide with lens paper

11. Animal Cell

12. Plant Cell

13. Chose a total of 6 prepared slides and draw at best magnification
Use oil for sperm and blood

14. 4x
10x
100x

15. Some slides may have more than one tissue type
Adipose
Hyaline cartilage

16. Pseudostratified ciliated columnar epithelium

17. Blood (RBC & WBC)
use oil

18. sperm
use oil

19. Cheek Cell
0.9% saline

20. Cheek Cell Scrape inside cheek
Swirl toothpick with cells in 0.9% saline
Place a small drop 0.01% methylene blue on saline
saline

21. Cheek Cell Place coverslip Observe under microscope Draw what you see
Clean up

22. Cheek Cell

23. Dispose Sharps

24. Clean Up

25. Microscope Parts Quiz

26. Microscope Quiz Which objective uses oil?
If your ocular is 10x and your objective is 40x what is the total magnification of your image?
Why do you need a cover slip and how do you avoid an air bubble?
Where do you dispose slides?