1. Autophagy Contributes to the neuroprotection of Nampt
Li Siying
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2. Glossary 1 Cerebral Ischemia 2 Autophagy 3
Nicotinamide phosphoribosyltransferase, Nampt
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3. Background Moderate Autophagy is neuroprotective1,2
Nampt is an endogenous protector against cerebral ischemia through inhibiting neuronal apoptosis and necrosis3
[1] Yue Z, Wang Q J, Komatsu M. Neuronal autophagy: going the distance to the axon[J]. Autophagy. 2008, 4(1):
[2] Shacka J J, Roth K A, Zhang J. The autophagy-lysosomal degradation pathway: role in neurodegenerative disease and therapy[J]. Front Biosci. 2008, 13:
[3] Wang P, Xu T Y, Guan Y F, et al. Nicotinamide phosphoribosyltransferase protects against ischemic stroke through SIRT1-dependent adenosine monophosphate-activated kinase pathway[J]. Ann Neurol. 2011, 69(2):
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4. Materials and Methods 1 2 3 8/30/2018
In vivo model: Middle cerebral artery occlusion (MCAO) in rats
In vitro model: Oxygen-glucose deprivation (OGD) in cultured cortical neurons
Nampt overexpression or knock-down by lentivirus-mediated gene transfering in vivo or in vitro
immunochemistry
electron microscope
immunoblotting assays
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5. Results
Figure1. Nampt overexpression up-regulates autophagy in the early phase of cerebral ischemia in rat MCAO model
图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1):
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6. Results
Figure2. Nampt regulates autophagy at the early phase of ischemic-like injury in cultured cortical neurons exposed to OGD stress
LV-shRNA-Nampt: Nampt knock down
图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1):
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7. Results
Figure3. Autophagy contributes to the neuroprotective effect of Nampt
3-MA, 3-methyladenine: autophagy inhibitor
rapamycin: autophagy inducer
图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1):
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8. Conclusion
图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1):
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9. Subsequent Results and Discussion
图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1):
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10. References
[1] Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1):
[2] Yue Z, Wang Q J, Komatsu M. Neuronal autophagy: going the distance to the axon[J]. Autophagy. 2008, 4(1):
[3] Shacka J J, Roth K A, Zhang J. The autophagy-lysosomal degradation pathway: role in neurodegenerative disease and therapy[J]. Front Biosci. 2008, 13:
[4] Wang P, Xu T Y, Guan Y F, et al. Nicotinamide phosphoribosyltransferase protects against ischemic stroke through SIRT1-dependent adenosine monophosphate-activated kinase pathway[J]. Ann Neurol. 2011, 69(2):
8/30/2018