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A. B. E64d+PA 2,5-5µg/ml C 2d 4d 6d SF + - LC3I LC3II GAPDH C 2d 4d 6d

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Presentation on theme: "A. B. E64d+PA 2,5-5µg/ml C 2d 4d 6d SF + - LC3I LC3II GAPDH C 2d 4d 6d"— Presentation transcript:

1 A. B. E64d+PA 2,5-5µg/ml C 2d 4d 6d SF + - LC3I LC3II GAPDH C 2d 4d 6d
BafA1 100nM SF LC3I LC3II + - GAPDH Fig S3. Time course of LC3 levels in LNCaP cells cultured in serum-free medium. LNCaP cells were grown in serum-containing medium (C) or serum-free medium (SF) for 2, 4 and 6 days. When indicated, cells were treated with (A) protease inhibitors E-64D and pepstatin A at a concentration of 2,5 μm/ml and 5 μm/ml, respectively or (B) 100 nM bafilomycin A1 for the last three hours in culture. Thereafter, cells were lysed and autophagy marker LC3 was measured in whole lysates by Western blot. GAPDH was used as a loading control. Representative Western blot images are shown.

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