1. Suppression by an h Current of Spontaneous Na+ Action Potentials in Human Cone and Rod Photoreceptors
Invest. Ophthalmol. Vis. Sci ;46(1): doi: /iovs
Figure Legend:
Human cones and rods expressed an SCN2 Na+ Channel. (A) The SCN2 Na+ channel was dominantly expressed in the human retina. Agarose gel electrophoresis of Na+ channel cDNAs obtained by RT-PCR from whole retina. Each pair of lanes shows the result from the primer set of SCN1A (1A), SCN2A (2A), SCN3A (3A), and SCN8A (8A) Na+ channels. The predicted size of each amplified Na+ channel cDNA was approximately 150 bp. Complementary DNA of the predicted size was detected only in the SCN2A lane. M, molecular weight marker; +, retinal cDNA; −, negative control (RNase-free water). (B) Human cones and rods express the SCN2 Na+ channel. Agarose gel electrophoresis of SCN2A cDNAs obtained by RT-PCR from single photoreceptor cells. Each lane shows the result from a single cone or rod. The predicted size of the amplified cDNA for SCN2A was approximately 160 bp, for GAPDH approximately 300 bp. In the cone and rod lanes, cDNA of the predicted size was detected in three of six cones and in one of two rods. N, no cell was added in RT-PCR (negative control); P, total retina was added in RT-PCR (positive control).
Date of download: 12/19/2017
The Association for Research in Vision and Ophthalmology Copyright © All rights reserved.