1. Microscopes in Depth Fall 2017
Microscopes & You
Microscopes in Depth
Fall 2017

2. Microscopes What we use! Magnifies organisms Types:
Light Microscopes
Electron Microscopes
MUCH higher magnification
Most powerful!
Scanning (SEM) & Transmission (TEM)
Magnification – increase in apparent size
Resolution –helps with clarity

3. Ocular lens (Eyepiece) Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Always carry a microscope with one hand holding the arm and one hand under the base.
Base

4. Structures & Function Microscope Part Function(s) Eyepiece
Contains the ocular lens; has a magnification of 10X
Revolving Nosepiece
Holds the high- and low- power objective lenses; can be rotated to change magnification.
Objective Lenses
There are three magnification lens: scanning (4X), low (10X), and high power (40X).
Stage Clips
Holds the slide in place.
Stage
Supports the slide as it is being viewed.
Light Source
Projects light upwards through the diaphragm, the specimen (what you’re looking at), and through the lenses
Diaphragm
Regulates the amount of light on the specimen.

5. Structures & Function Pt. 2
Microscope Part
Function(s)
Arm
Supports the entire microscope.
Fine Adjustment Knob
Moves the stage slightly to sharpen the image.
Coarse Adjustment Knob
Moves the stage up and down for focusing.
Base
Used to support the microscope when carried.

7. CHECK FOR UNDERSTANDING!
What knob would you use to enhance magnification?
What knob would you use to enhance resolution?
COARSE ADJUSTMENT KNOB
FINE ADJUSTMENT KNOB

8. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of
magnification?

9. Calculating Magnification
Eyepiece = 10x
Objective
Scanning = 4x (shortest in length)
Low Power = 10x
High Power = 40x (longest)
Magnification = Eyepiece x Objective
Field of view measured in microns
KNOW PARTS OF MICROSCOPE AND WHAT THEY DO!!

10. Power of Magnification
Band Color
Objective Power
Eyepiece Lens Power
Power of Magnification
Red 4x
10x
Yellow
Blue
40x
40x
100x
400x