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UCSF Core Facilities Enterprise

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Presentation on theme: "UCSF Core Facilities Enterprise"— Presentation transcript:

1 UCSF Core Facilities Enterprise
Jeff Milush, PhD Associate Professor Director UCSF/CFAR Core Immunology Laboratory Scientific Advisor to Laboratory of Clinical Virology November 17, 2017 CFAR Mentoring

2 What is a Core? A centralized shared resource Provides access to
Expert consultation Instruments Technologies Services Cell, animal, human support Cores come in many flavors, with varied operational models November 17, 2017 CFAR Mentoring

3 Why and when to use a Core?
Expand your research scope and capacity Expedite progress by using services already up and running Save money by ‘renting’ rather than ‘buying’ Gain critical insight by consulting with experts throughout the study Particularly at the study inception stage November 17, 2017 CFAR Mentoring

4 So how do I find the right Core?
~70 Cores at UCSF, 947 research resources Add more with affiliated institutions November 17, 2017 CFAR Mentoring

5 Gladstone Cores November 17, 2017 CFAR Mentoring

6 Blood Systems Research Institute (BSRI) Cores
November 17, 2017 CFAR Mentoring

7 November 17, 2017 CFAR Mentoring

8 November 17, 2017 CFAR Mentoring

9 What are the CFAR Scientific Cores?
Health Disparities Marguerita Lightfoot, PhD Clinical/Population Science Jeff Martin MD, MPH, Steve Deeks MD Immunology Jeff Milush PhD Pharmacology Fran Aweeka PharmD Specimen Banking John Greenspan BDS, PhD, Yvonne DeSouza Virology Paul Volberding, PhD Implementation SWG Elvin Geng, MD, MPH, Eric Goosby, MD November 17, 2017 CFAR Mentoring

10 Why are CFAR Cores special?
CFAR Cores exist to promote ‘Value Added’ services Education, training and mentoring State of the art equipment New assay development Customized services Streamlined interaction with other CFAR cores “Conception − to − Publication” support for projects November 17, 2017 CFAR Mentoring

11 Managing Core use can be daunting!
Specimens Nucleic Acid Extraction and Modification Bioinformatics HIV Sequencing Genomic SNP Analysis November 17, 2017 CFAR Mentoring

12 Core Synergy at Work! CFAR Clin and Specimen Core CTSI Biostats
CFAR Immunology Core BSRI Multiplex Assays Manuscript Grant Funding CFAR Virology Core UCSF Genomics Core November 17, 2017 CFAR Mentoring

13 Successful Core use through partnerships
Your role Picking a Core most relevant for the work needed Project ownership Clarifying your needs (timeline, materials, resources, budget) Defining project scope, complexity Core’s role Confirming it’s the right Core. If not, referral to others Established competency in all areas of service Interact with other cores if needed Defining deliverables (including timeline, defined services, budget, etc.) November 17, 2017 CFAR Mentoring

14 But can I afford using a core?
Consult with core director on study design, hypothesis, sample selection and services early in process You get what you pay for! Quality, accountability and experience Sometimes, time is $$ May partially cover costs for new development work Institutional supplements November 17, 2017 CFAR Mentoring

15 Remove barriers for 1st time core users
Pilot program up to $4,000 Remove barriers for 1st time core users Expand the scope and breadth of core users Spark new offerings and collaborations with cores Easy app, rapid turnaround, 6 month duration November 17, 2017 CFAR Mentoring

16 Core Immunology Laboratory

17 Core Immunology Laboratory Services
Services (tissue data): Cytometry Services: Consulting Analysis on LSR-II Customizable services User-Operated or Operator-Assisted Cell isolation from peripheral blood, leukapheresis or tissues (LN and gut) Sorting on ARIA Immunophenotyping, cell sorting, & viable cryopreservation Up to 17 color capability Training Immunomagnetic bead isolation Advanced Multicolor Cytometry Class Flow cytometric functional assays DiVA software course NK cell function 1:1 Data Analysis antigen-specific cytokine production degranulation Flow Clinic (Mondays 11-12) proliferation Contact us any time! RNAFlow Cytometry ELISA measures of cytokine and chemokine production Training in Immunologic techniques and data analysis

18 Core Virology Laboratory Services
Specimen Processing: Virologic Services: Consulting HIV-1 genotyping Cell isolation and cryopreservation from peripheral blood Int, RT-Pro RNA/DNA extraction from cells and tissue Dried blood spots Fluid processing and cryopreservation HIV-1 viral loads Plasma Sample shipping Cell Associated (RNA/DNA) Integrated DNA 2-LTR quantification TILDA (Tat/rev Induced Limiting Dilution Assay)

19 Questions? Jeffrey.Milush@ucsf.edu 206-3881
Thank You and Good Luck! Questions? November 17, 2017 CFAR Mentoring

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