1. PENTRA 60 C +

2. PENTRA 60

3. THROUGHPUT ANALYZES
CBC Mode /h
30 µl
CBC + 5Diff Mode /h
53 µl

4. PHYSICAL SPECIFICATIONS
Weight: 35 Kg
Height: 516 mm
Depth: 444 mm
Width: 481 mm

5. PHYSICAL SPECIFICATIONS
Power supplies:
from 100 Vac to 240 Vac
50 Hz to 60 Hz
Power Consumption:
Maximum 400 VA

6. Measurements and computation
Impedance for WBC, PLT, RBC, BASO
Photometry for Hgb
Impedance, light scattering and cytometry for : LYM, MON, NEU, EOS, ALY, LIC.
Computation from stored data that was directly measured for
Hct, MCV, MCH, MCHC, RDW, MPV, Pct, PDW.

7. TECHNOLOGY Multi-Distribution Sequential System MDSS (ABX Patent)
Double Hydrodynamic Sequential Syst. DHSS
Counting Principle IMPEDANCE
Permanent Rinse Flow PRF
SOLENOID VALVES ABX PATENT

8. System technology
MDSS
Sample Dilution Process

9. Sample delivery - Aspiration of 53 µl of whole blood
(5 Diff cycle) through the sampling
needle
- Whole blood split in 3 aliquots
injected successively in 3 tangential
flows of reagent

10. Sample Volumes

11. Step 1 : Sample Aspiration

12. Step 2 : Initial discard and rinse

13. Step3 : First Dilution

14. Step 4 : WBC/BASO Dilution

15. Step 5 : DIFF Dilution

16. Step 6 : Probe Wash

17. Step 7 : DIFF - Stop Reaction

18. Step 8 : RBC/Plt Aspiration

19. Step 9 : RBC/Plt Dilution

20. Step 10 : Hgb Dilution

21. Final Dilutions
First Dilution Diff chamber RBC/PLT chamber WBC/BASO chamber
Hgb chamber
10µl + 1.7ml Dil µl = 1ml Eosinofix µL Dln µl + 2 ml Alphalyse
- 42.5µl After 12 sec ml
+ 0.4 ml ml Dil
DILUTION DILUTION DILUTION DILUTION
1/ / / /200

22. Differential Dilution
25 µL Blood
µL Fix
µL Diluent
= 1/80
The EosinoFix reagent lyses the RBC, stabilizes the WBC in their native forms and stains the cells with a specific coloration, the Eosinophils are colored most intensely.
After 12 seconds reaction the solution is stabilized with Diluent and is then transferred to the flowcell for analysis.

23. General Characteristics of P60 C+ User Interface
Pilots all the analysers’ cycles: analysis, regulation, maintenance and grinding cycles.
Calculates a result from the analyser measurements, pathology generation.
Follow-up system of the analyser reagent quantities.
Full view of result in colour
Management of a work list to predetermine analysis to be carried out.

24. General Characteristics of P60 C+ User Interface
Stat analysis function.
Management of a barcode reader to identify samples.
Stocking of results, with consultation and research carried out by identification
Validation and re-run of the sample on request of the user.
Maintenance and regulation functions of analyser.
Parameters allowing the user to adapt the ASP01 to its needs.
Managment of papers ensuring tracability of sensitive actions.

25. General Characteristics of P60 C+ User Interface
QC: Quality Control.
Calibration of analyser
XB: patient quality control.
Repeatability.
Connection with a host computer system for reception of files and emission of results.
Printing.

26. CBC Result CBC

27. CBC/DIFF result