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The polymerase chain reaction

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Presentation on theme: "The polymerase chain reaction"— Presentation transcript:

1 The polymerase chain reaction
CHAPTER TWENTY-THREE The polymerase chain reaction

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4 Figure 23.01

5 Figure 23.04

6 Figure 23.05

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9 Figure 23.02

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11 Value for indicated DNA polymerase
Property Value for indicated DNA polymerase KOD DNA polymerase Pfu DNA polymerase Taq DNA polymerase Origin Archaea Bacteria Deduced molecular mass (kDa) 90.0 90.1 93.9 Optimum temperature (ºC) 75 Optimum pH at 75ºC 6.5 Thermostability (half-life) 95ºC,12 h; 100ºC,3.0 h 95ºC, 6h; 100ºC, 2.9h 95ºC, 1.6 h 5'→3' exonuclease activity - + 3'→5' exonuclease activity Terminal transferase activity Processivity (bases) >300 <20 ND Elongation rate (bases/s) 25 61

12 Reverse transcription PCR

13 Figure 23.15

14 Figure 23.16

15 Figure 23.08

16 PCR for cloning

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18 One-step assembly PCR fusion PCR

19 PCR for detection

20 Real-time fluorescent PCR

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22 Figure 23.25

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