1. LABORATORY EXERCISES FOR MALE REPRODUCTIVE SYSTEM

2. Slide #101 (1096). Testis, rat. sustentacular (“Sertoli”) cells
pale nuclei of these cells are visible adjacent to the limiting membrane of the seminiferous tubules
Nuclei of Sustentacular cells
THE SERTOLI CELLS (SUSTENTACULAR CELLS) EXTEND THE FULL THICKNESS OF THE WALL OF
THE SEMINIFEROUS TUBULE. IN OTHER WORDS, THE SERTOLI CELLS EXTEND FROM THE BASEMENT MEMBRANE TO THE LUMEN OF THE SEMINIFEROUS TUBULE.
THE FUNCTION OF THE SERTOLI CELL IS SUPPORT FOR THE DEVELOPING GERM CELLS.
Leydig
cells
Spermatogonia
Spermatocytes
Spermatids

3. Horse
Sperm
This EM at

4. Slide #101 (1096). Testis, rat.
Nuclei of sustentacular or Sertoli cells
Myoid cell nucleus
Spermatogonia
Spermatocytes
Spermatids
seminiferous
tubule lumen
Spermatocyte
Leydig
cells

5. Slide #101 (1096). Testis, rat. Primary S. Secondary S. spermatid
Meiotic figures
(metaphase plates)
spermatids
seminiferous
tubule
WHEN A SPERMATOGOIUM CELL IS SIGNALED TO UNDERGO MEIOSIS INSTEAD OF MITOSIS, THE CELL HAS REPLICATED ITS DNA TO BECOME 4N AND HAS ENTERED INTO THE FIRST PHASE OF MEIOSIS BUT NOT YET DIVIDED, SO THAT IT IS A DIPLOID 4N CELL, WHICH IS CALLED A PRIMARY (1°) SPERMATOCYTE.
Myoid cell
Spermatogonia
Spermatocytes
Sustentacular cell nuclei
or Sertoli cells
Leydig
cells
Secondary
spermatocytes

6. Mitotic metaphase Meiotic metaphase I Meiosis Mitosis Spermatogonia
base
Meiotic
Metaphase II
Mitotic
metaphase
Meiotic metaphase I
Secondary spermatocytes
lumen
Human testis Epon toluidine blue stain

7. SEMINIFEROUS TUBULES COMPOSED OF:
SPERMATIDS
Primary SPERMATOCYTES
SERTOLI CELLS
MYOID CELL
nucleus
GERM CELLS
SPERMATOGONIA,
SPERMATOCYTES,
SPERMATIDS
Human testis

8. human

9. Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.
seminiferous
tubules
rete testis and
mediastinal testis
Testis
epididymis
Leydig cells produce TESTOSTERONE
interstitial tissue
between the
seminiferous
tubules is quite thin.
ductus deferens (tail of epididymis)

10. Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.
CCT capsule’s proper
TUNICA ALBUGINIA
The interstitial tissue
between the
seminiferous
tubules is quite thin
Leydig cells produce
TESTOSTERONE

11. Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.
Sertoli cells; spermatogonia; primary spermatocytes; and early and late spermatids
spermatogonia;
Secondary spermatocytes

12. Golgi phase
Cap phase
Elongation phase
Maturation phase

13. DEMO SLIDE BOX 100 – Testis, stallion.
Sertoli cells;
spermatogonia;
primary spermatocytes;
late spermatids
Leydig cells
sustentacular
(“Sertoli”) cells;
LEYDIG (INTERSTITIAL) CELLS ARE LARGE, ROUND TO OVAL CELLS WITH ACIDOPHILIC BUT OFTEN PALELY STAINING CYTOPLASM. SOMETIMES THESE CELLS CAN APPEAR FOAMY DUE TO THE FACT THAT THEY ARE STEROID PRODUCING CELLS.

14. DEMO SLIDE BOX 99 –Testis, newborn kitten.
Nuclei of
sustentacular cells
epididymis
rete testis and
mediastinal testis
gonocytes

15. DEMO SLIDE BOX 226 – (PM-172 b). Testis, epididymis, ductus deferens, pig (immature).
rete testis and
mediastinal testis
epidermidis
Dividine gonocytes
gonocytes
Nuclei of sustentacular (“Sertoli”) cells;

16. DEMO SLIDE BOX 187 (935). Testis, boar.
DEGENERATION and loss of germ cells in the seminiferous tubules; however, it illustrates the open spaces between tall Sertoli cells where germ cells would have been located as indicated in the drawing of Sertoli cells
Slide #107
Normal cat testis
IF THE MALE IS A BILATERAL CRYPTORCHID THEN HE WILL BE INFERTILE; IF THE MALE IS A UNILATERAL CRYPTORCHID THEN HE likely WILL HAVE REDUCED FERTILITY.
Cryptorchid testis

17. DEMO SLIDE BOX 189 (985) -Epididymis, goat.
ductuli
efferentes.
ductuli aberrantes
vestigial
mesonephric tubules
ductus epididymidis.

18. DEMO SLIDE BOX 224 – (641). Epididymis, boar.
ductuli efferentes
ductus epididymidis,

19. DEMO SLIDE BOX 101 –Ductus deferens, stallion.
thick tunica muscularis
epithelium and lamina propria/submucosa
smooth muscle

20. Slide #119 (Ed 904-160c). Ampulla, donkey.
proteinaceous material
In lumen of the ampulla.
simple or stratified cuboidal epithelium
CCT and smooth muscle.

21. DEMO SLIDE BOX 102 – (998.) Ampulla of ductus deferens, stallion.
simple or stratified cuboidal epithelium
Smooth muscle

22. Slide #126 (Dog 1-160-1). Prostate, dog.
urethral lumen.
TRANSITIONAL
simple cuboidal or simple columnar (like the prostate) and doesn’t have stereocilia.

23. DEMO SLIDE BOX 105 – Prostate gland, dog.
fibromuscular tissue
Urethra with
transitional
epithelium
small ampullae of
the ductus deferens

24. DEMO SLIDE BOX 103 – Prostate gland (pars disseminata), bull
principal duct of the vesicular glands
or ductus deferens;
urethra
SKELETAL MUSCLE, WHICH IS CALLED THE URETHRALIS MUSCLE OR THE EXTERNAL URETHRAL SPHINCTER

25. Slide #131 (946) -- Bulbourethral gland, boar.

26. Slide #137 (Ed 904-173B). Vesicular gland, stallion.
seminal vesicles because they are hollow, blind sacs
vesicular glands or seminal vesicles

27. DEMO SLIDE BOX 191–(991) Bulbourethral gland, goat.
mucous character
skeletal muscle fibers of the bulbospongiosus muscle.

28. DEMO SLIDE BOXES 107– Penis, dog.
pectiniform septum - separates the paired corpora cavernosum
tunica albuginea (of the penis CCT)
urethra -TRANSITIONAL
PRIMARILY SMOOTH MUSCLE ( YOU MAY SEE A FEW SKELETAL MUSCL FIBERS MIXED IN WITH THE SMOOTH MUSCLE – ESPECIALLY PROXIMALLY
dorsal penile artery, vein & nerve-
MUSCULAR ARTERY

29. DEMO SLIDE BOXES 108 – Penis, dog.

30. DEMO SLIDE 193 –(1086). Penis, dog.
the glans penis and includes part of the prepuce
internal lamina of the prepuce
preputial cavity
skin of the penis
pars longa glandis - peripherally located
erectile tissue
urethra
corpus spongiosum - erectile tissue surrounding the urethra
os penis

31. DEMO SLIDE BOX 192 (1054). –Penis, bull calf.
corpus cavernosum penis,
urethra,
corpus spongiosum.
skin of the penis and
internal lamina of the prepuce are fused at this location

32. Slide #56 (Pm1-166C-B1). Penis, boar (immature).
corpus cavernosum penis, the urethra, and the corpus spongiosum.
nerve, collagenous connective tissue (CCT), large vein, arterioles, venules, capillaries.
nerve
Small muscular artery,
Unilocular
adipose
Tissue (white fat),

33. corpus cavernosum penis
277
Human Penis – transitional epithelium and surrounding spongy cavernous of penal urethra
corpus cavernosum penis
urethra
nerve
Transitional epithelium of urethra
Corpus spongiosum
Smooth muscle
Tunica albuginea (of the penis, CCT)
Corpus spongiosum
skin