1. Fast Electrical Impedance Spectroscopy measurement techniques for dynamic bioimpedance characterization. Ph.D. Student: B.Sánchez Thesis Advisor: R.Bragós
Electronic Engineering Department
Universitat Politècnica de Catalunya

2. Contents
1.- Introduction
2.- Motivation
3.- Multitone Burst Signal
4.- Results
5.- Conclusions

3. Introduction Linear Time Invariant system: Impedance Transfer Function
Laplace/Fourier Transform 1

4. Introduction Biological systems: Bioimpedance 2 Equivalent Circuit
Cell membrane
Extracellular matrix LF HF f R 8
|Z| Ro fc 2

5. Introduction
Ex-vivo tissue characterization: 3

6. Introduction
In-vitro static cell culture growth characterization: 4

7. Motivation
Challenge (I): In-vitro myocites cell culture growing characterization for tissue engineering applications
Link video 5

8. Motivation
Challenge (II): In-vivo myocites patch monitoring and characterization: 6

9. Multitone Burst Signals
Time domain: 7

10. Multitone Burst Signals
Frequency domain: 8

11. Multitone Burst Signals
Crest Factor:
Newman:
Schroeder:
Genetic Algorithm: 9

12. Multitone Burst Signals
Frequency Plan:
Avoid intermodulation products: shift original tones for BW guard. 10

13. Multitone Burst Signals
Multitone bursts with Bilateral Quasi Logarithmic Frequency Distribution: 11

14. Results Measurement Hardware Setup:
Custom FPGA based arbitrary signal generator.
DAC 12bits, 16Msamples, 40Ms/s, 1st order hold.
Ethernet capable controller.
PXI solution
PXI-5422: AWG, 200Ms/s, 32MB,16 bits.
PXIe-5122: AD 2channels, 100Ms/s, 64MB/channel, 14bits.
PXI-2530: multiplexation matrix, 128 channels. 12

15. Results Yeast cell suspension settlement characterization:
Estimation of the Growth Cell Curve 13

16. Conclusions
Non invasive and non destructive technique for passive electrical properties charaterization of biological materials.
Valid measurement method for cell growth estimator and suitable for dynamic biological materials and systems characterization.
Wide application areas: electroporation, tomography, cell counting in microfludic, biosensors, tissue engineering,..
The final application field is the cardiac tissue characterization in regenerative medicine applications. 14

17. Fast Electrical Impedance Spectroscopy measurement techniques for dynamic bioimpedance characterization. Ph.D. Student: B.Sánchez Thesis Advisor: R.Bragós
Electronic Engineering Department
Universitat Politècnica de Catalunya