1. Tools of a Biologist

2. Dissecting Microscope
Microscopes
Electron Microscope
Light Microscope
Dissecting Microscope

3. Separating Molecules
Centrifuge
Chromatography
Gel Electrophoresis

4. Mixing Liquids
Lab Blender
Vortex
Mixer
Magnetic
Stirrer

5. Measuring Length

6. Metric ruler, Meter Stick, Measuring Tape

7. Measuring mass

8. Weighing boats & weighing paper

9. Balance Steps: 1. Press ON
2. Place Weighing Boat/Weighing Paper/Beaker on the balance
Press ZERO (Tare)
4. Wait for the reading to stabilize and record
5. Leave the balance on until the end of the lab

10. Measuring Volume

11. Beakers & Flasks PROS: Measure large volumes quickly Great for storage
Good for chemical reactions
CONS:
VERY Inaccurate

12. Always use a test tube rack
Test tubes
PROS:
Great for small-scale chemical reactions or for preparing small volumes of chemicals
CONS:
No measurement
Very fragile
Always use a test tube rack

13. Graduated cylinders PROS: Accurate Measurements CONS:
Not for chemical reactions
Reading meniscus can be challenging
Fragile

14. Beral Pipettes (Transfer Pipettes)
PROS:
Fairly Accurate
Disposable
CONS:
Can be hard to measure precisely
1mL
0.75mL
0.5mL
0.25mL

15. Serological Pipettes (graduated Pipettes)
PROS:
Very Accurate Measurements
Disposable
CONS:
Can be cumbersome and requires good manual dexterity

16. How to use a Pipette Pump
Plunger
Thumb Wheel
Release Lever
Steps:
Firmly insert the flat end of the pipette into the pump 1mL Pipette = Green Pump 5mL, 10mL, 25mL Pipettes = Pink Pump
Place the tip of the pipette into the liquid being measured
Rotate thumb wheel down to draw up the correct volume
Measurements are done at eye level
Your other hand should support the pipette
Press and hold the release lever to dispense the liquid in the desired container
Rotate the thumb wheel up to dispense any remaining liquid
DO NOT LEAVE THE PUMP ON THE PIPETTE!!!!!

17. Micropipettes PROS: Very Accurate Small Measurements Disposable Tips
CONS:
Can be cumbersome and requires good manual dexterity