Presentation is loading. Please wait.

Presentation is loading. Please wait.

FIG. 1. Comparison of luciferase signal in ZFL, ZELH, ZELH-zfERα, ZELH-zfERβ1, and ZELH-zfERβ2 cells either left untreated (DMSO control) or exposed to.

Published byEmory Garrison Modified over 6 years ago

Similar presentations

Presentation on theme: "FIG. 1. Comparison of luciferase signal in ZFL, ZELH, ZELH-zfERα, ZELH-zfERβ1, and ZELH-zfERβ2 cells either left untreated (DMSO control) or exposed to."— Presentation transcript:

1 FIG. 1. Comparison of luciferase signal in ZFL, ZELH, ZELH-zfERα, ZELH-zfERβ1, and ZELH-zfERβ2 cells either left untreated (DMSO control) or exposed to E2. Relative luminescent units were expressed for 25,000 cells/per well. Results are means of triplicates ± SD; *Statistically different from DMSO control within the cell line (p < 0.05). From: Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line Toxicol Sci. 2011;125(2): doi: /toxsci/kfr297 Toxicol Sci | © The Author Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please

2 FIG. 2. Concentration-dependent luciferase induction in response to 17β-estradiol (E2) in ZFL cells either transiently (solid motifs/dash line) or stably (open motif/plain line) transfected with zfER subtypes and ERE-βGlob-Luc-SV-hygro. Luciferase activity is expressed as percentage of maximal luciferase induction by E2 10nM in ZELH-zfERα cells and E2 3nM in ZELH-zfERβ1 and ZELH-zfERβ2 cells. Results are means of triplicates ± SD and are representative of three (transient) or 14–15 (stable) independent experiments. From: Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line Toxicol Sci. 2011;125(2): doi: /toxsci/kfr297 Toxicol Sci | © The Author Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please

3 FIG. 3. Expression of zfERα, zfERβ1, and zfERβ2 mRNA in stably transfected ZELH-zfERs and parental ZFL cell lines as detected by real-time PCR. Results are expressed as relative levels of zfER mRNA level in stably transfected cells over that measured in parental ZFL cell line. Results (means ± SD) are representative of two independent experiments. From: Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line Toxicol Sci. 2011;125(2): doi: /toxsci/kfr297 Toxicol Sci | © The Author Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please

4 FIG. 4. Effect of E2 on cell proliferation in ZELH-zfERα, ZELH-zfERβ1, and ZELH-zfERβ2 cell lines, as determined by MTT coloration test. Results are expressed as % of DMSO-treated cells means ± SD of triplicates and are representative of three independent experiments; *Statistically different from DMSO control (p < 0.05). From: Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line Toxicol Sci. 2011;125(2): doi: /toxsci/kfr297 Toxicol Sci | © The Author Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please

5 FIG. 5. Dose response curves of luciferase induction in ZELH-zfERα by various ER ligands: (A) natural estrogens: 17β-estradiol (E2), estrone (E1), and estriol (E3); industrial chemicals: 4-tert-octylphenol (4tOP), bisphenol A (BPA), and 1,1,1-trichloro-2-(2-chlorophenyl)-2-(4-chlorophenyl)ethane (o,p′-DDT) (B) pharmaceutical compounds: diethylstilbestrol (DES), hexestrol (Hex), and 17α-ethynylestradiol (EE2), (C) α-zearalenol (α-Zee), β-zearalenol (β-Zee), α-zearalanol (α-Zea), and genistein (Gen), (D) benzophenone derivates: 2,4-dihydroxybenzophenone (benzophenone 1 or BP1), 2,2’,4,4’-tetrahydroxybenzophenone (benzophenone 2 or BP2), and 2,4,4’-trihydroxybenzophenone (THB). Cells were exposed for 72 h; values are expressed as percentage of luciferase induction by E2 10nM (means ± SD of triplicates). From: Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line Toxicol Sci. 2011;125(2): doi: /toxsci/kfr297 Toxicol Sci | © The Author Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please

6 FIG. 6. Dose response curves of luciferase induction in ZELH-zfERβ1 by various ER ligands: (A) natural estrogens: 17β-estradiol (E2), estrone (E1), and estriol (E3), (B) pharmaceutical compounds: diethylstilbestrol (DES), hexestrol (Hex), and 17α-ethynylestradiol (EE2), (C) α-zearalenol (α-Zee), β-zearalenol (β-Zee), α-zearalanol (α-Zea), and genistein (Gen), (D) benzophenone derivates: 2,4-dihydroxybenzophenone (benzophenone 1 or BP1), 2,2’,4,4’-tetrahydroxybenzophenone (benzophenone 2 or BP2), and 2,4,4’-trihydroxybenzophenone (THB). Cells were exposed for 72 h; values are expressed as percentage of luciferase induction by E2 3nM (means ± SD of triplicates). From: Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line Toxicol Sci. 2011;125(2): doi: /toxsci/kfr297 Toxicol Sci | © The Author Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please

7 FIG. 7. Dose response curves of luciferase induction in ZELH-zfERβ2 by various ER ligands: (A) natural estrogens: 17β-estradiol (E2), estrone (E1), and estriol (E3); industrial chemicals: 4-tert-octylphenol (4tOP), bisphenol A (BPA), and 1,1,1-trichloro-2-(2-chlorophenyl)-2-(4-chlorophenyl)ethane (o,p′-DDT) (B) pharmaceutical compounds: diethylstilbestrol (DES), hexestrol (Hex), and 17α-ethynylestradiol (EE2), (C) α-zearalenol (α-Zee), β-zearalenol (β-Zee), α-zearalanol (α-Zea), and genistein (Gen), (D) benzophenone derivates: 2,4-dihydroxybenzophenone (benzophenone 1 or BP1), 2,2’,4,4’-tetrahydroxybenzophenone (benzophenone 2 or BP2), and 2,4,4’-trihydroxybenzophenone (THB). Cells were exposed for 72 h; values are expressed as percentage of luciferase induction by E2 3nM (means ± SD of triplicates). From: Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line Toxicol Sci. 2011;125(2): doi: /toxsci/kfr297 Toxicol Sci | © The Author Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please

Download ppt "FIG. 1. Comparison of luciferase signal in ZFL, ZELH, ZELH-zfERα, ZELH-zfERβ1, and ZELH-zfERβ2 cells either left untreated (DMSO control) or exposed to."

Similar presentations

B Supplementary Fig S1. (A) ZR75- and MCF7-PELP1 knockdown cells were generated as described in methods section. Pooled colonies were analyzed for PELP1.

B Supplementary Fig S1. (A) ZR75- and MCF7-PELP1 knockdown cells were generated as described in methods section. Pooled colonies were analyzed for PELP1.
From: An open day in the metric space

From: An open day in the metric space
FIG. 1. Scanning electronic microscope image of the unmodified beads; all the bead samples were identical in appearance and size to the unmodified shown.

FIG. 1. Scanning electronic microscope image of the unmodified beads; all the bead samples were identical in appearance and size to the unmodified shown.
Figure 1 WBC count (A), platelet count (B), MPV (C), platelet activation rate (D) and TGF-β1concentration (E) in the PF of women with endometriosis and.

Figure 1 WBC count (A), platelet count (B), MPV (C), platelet activation rate (D) and TGF-β1concentration (E) in the PF of women with endometriosis and.
Figure 6. Biochemical analysis of mTORC1- and mTORC2-dependent signaling in response to UVB. iRicKO cells were treated with vehicle or with 4OHT for 3.

Figure 6. Biochemical analysis of mTORC1- and mTORC2-dependent signaling in response to UVB. iRicKO cells were treated with vehicle or with 4OHT for 3.
Fig. 6 Gene expression and DNA methylation in callus

Fig. 6 Gene expression and DNA methylation in callus
Fig. 1 Flow chart of the COBRA-light trial and its extension study

Fig. 1 Flow chart of the COBRA-light trial and its extension study
Figure 6. Effect of Withaferin A (WA) treatment on leptin-induced signaling in breast cancer cells. A) Immunoblotting for pSTAT3 (Tyr705), total STAT3,

Figure 6. Effect of Withaferin A (WA) treatment on leptin-induced signaling in breast cancer cells. A) Immunoblotting for pSTAT3 (Tyr705), total STAT3,
From: Global Banking: Recent Developments and Insights from Research*

From: Global Banking: Recent Developments and Insights from Research*
(A) The binding between purified prothrombin and V5-His-RPN2 (filled circle) was confirmed by ELISA. BSA (filled triangle) was used as the control. The.

(A) The binding between purified prothrombin and V5-His-RPN2 (filled circle) was confirmed by ELISA. BSA (filled triangle) was used as the control. The.
FIG. 1. H2S contents in plasma and myocardial tissue were measured by the Methylene Blue method (A, B). Representative immunoblots and densitometric.

FIG. 1. H2S contents in plasma and myocardial tissue were measured by the Methylene Blue method (A, B). Representative immunoblots and densitometric.
Fig. 1 Graphical representation

Fig. 1 Graphical representation
Figure 1. Overexpression of Kiss1r- or Adcyap1r-expressing vectors in LβT2 cells. LβT2 cells were transfected with 2.0 μg of Kiss1r-expressing (A) or Adcyap1r-expressing.

Figure 1. Overexpression of Kiss1r- or Adcyap1r-expressing vectors in LβT2 cells. LβT2 cells were transfected with 2.0 μg of Kiss1r-expressing (A) or Adcyap1r-expressing.
Figure 5. Targeted inhibition of DNMT enzymes reduces the fibrotic markers collagen and ASMA. The impact of reducing DNMT levels on the expression of fibrosis-related.

Figure 5. Targeted inhibition of DNMT enzymes reduces the fibrotic markers collagen and ASMA. The impact of reducing DNMT levels on the expression of fibrosis-related.
Fig. 1 UGT2B15 mRNA levels are stimulated by E2 in a time- and dose-dependent manner in MCF-7 breast cancer cells. A, Time course for E2 treatment. Cells.

Fig. 1 UGT2B15 mRNA levels are stimulated by E2 in a time- and dose-dependent manner in MCF-7 breast cancer cells. A, Time course for E2 treatment. Cells.
From: Political Leadership and Power Redistribution

From: Political Leadership and Power Redistribution
Glutamic acid ameliorates estrogen deficiency–induced menopausal-like symptoms in ovariectomized mice  Na-Ra Han, Hee-Yun Kim, Woong Mo Yang, Hyun-Ja.

Glutamic acid ameliorates estrogen deficiency–induced menopausal-like symptoms in ovariectomized mice  Na-Ra Han, Hee-Yun Kim, Woong Mo Yang, Hyun-Ja.
Figure 1. The Modified Dual Pathway Model.

Figure 1. The Modified Dual Pathway Model.
Fig. 1 Increased MIR155HG expression correlates with glioma grade and mesenchymal transition and confers a poor prognosis in GBM patients. (A) The level.

Fig. 1 Increased MIR155HG expression correlates with glioma grade and mesenchymal transition and confers a poor prognosis in GBM patients. (A) The level.
Figure 1. WA down-regulates H

Figure 1. WA down-regulates H

Similar presentations

Ads by Google